Histidine‑Stabilized Copper Nanoclusters as a Fluorescent Probe for Selective and Sensitive Determination of Vitamin B_(12)  

作　　者：Krishnamoorthy Shanmugaraj Thangarasu Sasikumar Malaichamy Ilanchelian 

机构地区：[1]Centre for Nanoscience and Nanotechnology,Department of Chemistry,The Gandhigram Rural Institute,Gandhigram,Dindigul,Tamil Nadu 624302,India [2]Department of Chemistry,Bharathiar University,Coimbatore,Tamil Nadu 641046,India

出　　处：《Journal of Analysis and Testing》2018年第2期168-174,共7页分析检测（英文）

基　　金：K.Shanmugaraj acknowledges the DST-SERB,New Delhi,India,for the award of the SERB National Post-Doctoral Fellowship(File Number:PDF/2016/002801);T.Sasikumar acknowledges the University Grants Commission(UGC)-BSR Research Fellowship(JRF)Government of India for the financial support(F.25-1/2014-15(BSR)7-26/2007/(BSR)dated:05.11.2015);The authors are grateful to the PSG Institute of Advanced Studies,Coimbatore,for HR-TEM measurements.

摘　　要：Herein,we report the highly sensitive and selective determination of vitamin B_(12)(VB_(12))using histidine-stabilized copper nanoclusters(His-CuNCs)as a fluorescent probe by the spectrofluorometry method.The as-synthesized His-CuNCs were characterized by UV–Vis absorption,steady-state emission,and high-resolution transmission electron microscopy measurements.His-CuNCs exhibited an emission maximum around 443 nm,when excited at 350 nm.The emission intensity of His-CuNCs was significantly quenched by the addition of VB_(12),hinting that the synthesized probe can be employed for sensing of VB_(12).Based on the decrease in emission intensity,the concentration of VB_(12) was determined and the emission intensity of His-CuNCs can be quenched by VB_(12) via fluorescence resonance energy-transfer mechanism.His-CuNCs showed high selectivity toward the determination of VB_(12) in the presence of other potentially interfering vitamins,cations,and anions.In addition,the proposed sensing platform displayed a sensitive response to VB_(12) in the linear range of 4.00–28.00×10^(−6) mol dm^(−3) with a limit of detection of 3.30×10^(−9) mol dm^(−3).The proposed sensing system is simple,low cost,selective,and sensitive for the determination of VB_(12) in biological samples.

关 键 词：Histidine-stabilized copper nanoclusters Energy transfer SPECTROFLUOROMETRY Emission quenching Vitamin B_(12) 

分 类 号：O64[理学—物理化学]