长沙地区南瓜白粉病病原菌及种质资源抗性的鉴定  

Identification of squash powdery mildew pathogen in Changsha and resistance germplasm screening

作　　者：严佳丽 楚箫 黎豪 梁少华 孙小武 YAN Jiali;CHU Xiao;LI Hao;LIANG Shaohua;SUN Xiaowu(College of Horticulture,Hunan Agricultural University/Engineering Research Center for Horticultural Crop Germplasm Creation and New Variety Breeding,Ministry of Education,Changsha 410128,Hunan,China)

机构地区：[1]园艺作物种质创新与新品种选育教育部工程研究中心·湖南农业大学园艺学院,长沙410128

出　　处：《中国瓜菜》2022年第4期20-26,共7页China Cucurbits And Vegetables

基　　金：国家重点研发计划(2018YFD0100700);湖南省重点研发项目(2020NK2038)。

摘　　要：为鉴定湖南省长沙市芙蓉区金山基地南瓜白粉病病原菌种类及生理小种,运用显微形态观察、分子生物学鉴定及国际通用的13个甜瓜生理小种鉴别寄主和鉴别标准体系等方法,对该地区的南瓜白粉病病原菌进行鉴定,并对90份南瓜种质资源的抗病性进行鉴定。结果表明,长沙地区南瓜白粉病病原菌为单囊壳白粉菌(Podosphaera xanthii),生理小种为2France;筛选出9份高抗种质,均为中国南瓜类型;筛选出20份高感种质,其中7份为中国南瓜类型,13份为印度南瓜类型。明确了长沙地区的白粉病病原菌种类及其生理小种,筛选出高抗白粉病的南瓜种质资源,为选育抗白粉病的南瓜新品种及抗病基因定位研究奠定了基础。To identify powdery mildew physiological races at Jinshan station of Furong District, Changsha City, Hunan Province, the pathogens of squash powdery mildew were assayed by means of micromorphological observation,molecular biology identification and differential host testing, the 13 international commonly used melon differentials.Ninety squash accessions, both Cucurbita moschata and Cucurbita maxima, were tested for resistance to the pathogen.Our results suggested that the squash powdery mildew pathogen in this area was P. xanthii race 2France. Among the tested accessions 9 were highly resistant and were C. moschata. Of the 20 highly susceptible accession 7 were C. moschata and 13 were C. maxima. Our research determined the squash powdery mildew pathogen in Changsa and identified resistance germplasm. This laid foundation for resistant squash breeding and gene localization.

关 键 词：南瓜 白粉病 生理小种鉴定 抗病种质 

分 类 号：S642.1[农业科学—蔬菜学]