延迟放瓶对2种自动化血培养系统苛养菌培养的影响  

Influence of delayed entry on fastidious bacterium culture in 2 blood culture systems

作　　者：褚敏君 李小娟[1] 黄海林[1] 李珏 麻明彪 杜廷义[1] 王海平 CHU Minjun;LI Xiaojuan;HUANG Hailin;LI Jue;MA Mingbiao;DU Tingyi;WANG Haiping(Department of Clinical Laboratory,Kunming Children's Hospital,Children's Hospital Affiliated to Kunming Medical University,Kunming 650228,Yunnan,China)

机构地区：[1]昆明市儿童医院昆明医科大学附属儿童医院检验科,云南昆明650228

出　　处：《检验医学》2022年第3期209-212,共4页Laboratory Medicine

基　　金：云南省教育厅科学研究基金资助项目(2021Y348);云南省教育厅科学研究基金资助项目(2021J028);昆明市卫生科技人才培养项目暨“十百千”工程培养计划[2016-SW(省)-20];昆明市卫生科技人才培养项目暨“十百千”工程培养计划[2020-SW(后备)-109]。

摘　　要：目的比较延迟放瓶对2种自动化血培养系统苛养菌阳性检出率和检出时间的影响。方法用肺炎链球菌和流感嗜血杆菌标准菌株制成不同浓度菌液模拟样本,分别接种到BactecPeds Plus和BACT/ALERT PF培养瓶中,室温条件下放置0、8、16、24 h后,分别放入BACTEC FX血培养仪和BACT/ALERT 3D血培养仪,记录培养结果和报阳时间,并进行统计分析。结果室温放置不同时间后,不同浓度菌液模拟样本在2种血培养仪中均可检测到阳性结果,BACTEC FX血培养仪报阳时间显著短于BACT/ALERT 3D血培养仪(P<0.001)。肺炎链球菌室温放置8、16和24 h后,报阳时间均显著长于放置0 h(P<0.05),且延迟放瓶时间与菌液浓度、血培养系统2个因素之间均存在交互作用(P<0.05)。流感嗜血杆菌延迟放瓶0和8 h的报阳时间差异无统计学意义(P>0.05),且延迟放瓶时间与菌液浓度、血培养系统2个因素之间无交互作用(P>0.05)。结论苛养菌模拟样本报阳时间随延迟放瓶时间的延长呈增加趋势,这一趋势受到菌种、菌液浓度、血培养系统等因素的多重影响。Objective To compare the effects of delayed entry on the positive detection rate and time of fastidious bacterium culture in 2 blood culture systems.Methods Simulated blood samples of Streptococcus pneumoniae and Haemophilus influenzae standard isolates with different concentrations were inoculated into BactecPeds Plus and BACT//ALERT PF culture flask at room temperature for 0,8,16 and 24 h,and then they were placed into the above 2 blood culture systems(BACTEC FX and BACT/ALERT 3D).The culture results and positive times were recorded for statistical analysis.Results After being placed for different periods,positive results were determined in the all simulated blood samples,and the positive time of BACTEC FX was shorter than that of BACT/ALERT 3D(P<0.001).After the delayed entry for 8,16 and 24 h,the positive time of Streptococcus pneumoniae was longer than that of the sample placed immediately(0 h)(P<0.05).There were interactions between the delayed entry time,and the concentration,the blood culture systems(P<0.05).There was no statistical significance in the positive time of Haemophilus influenzae between 0 h and 8 h of delayed entry(P>0.05),and the delayed entry time,and the concentration,the blood culture systems had no interaction(P>0.05).Conclusions The positive time of fastidous bacterium increases with the extension of the delayed entry time,which was influenced by multiple factors,such as strain,concentration and blood culture system.

关 键 词：苛养菌 血培养 模拟样本 延迟放瓶 

分 类 号：R446.5[医药卫生—诊断学]