lncRNA SNHG5对人类下咽癌细胞转录组学的影响及意义  

Effect of long non-coding RNA SNHG5 on the transcriptome of human hypopharyngeal carcinoma cells and clinical significance

作　　者：周焕 唐颖 杜翠萍[2] 杨扬[2] 冯会杰[2] 邱淼欣 闫佩毅[3] 蔡骁垚 金姝 ZHOU Huan;TANG Ying;DU Cuiping;YANG Yang;FENG Huijie;QIU Miaoxin;YAN Peiyi;CAI Xiaoyao;JIN Shu(Hengyang Medical School,University of South China,Hengyang 421001,Hunan,China;Department of Otolaryngology,Shanghai Putuo District People's Hospital,Shanghai 200060,China;Department of Clinical Laboratory,Shanghai Putuo District People's Hospital,Shanghai 200060,China;Department of Central Laboratory,Shanghai Putuo District People's Hospital,Shanghai 200060,China)

机构地区：[1]南华大学衡阳医学院,湖南衡阳421001 [2]上海市普陀区人民医院耳鼻喉科,上海200060 [3]上海市普陀区人民医院检验科,上海200060 [4]上海市普陀区人民医院中心实验室,上海200060

出　　处：《检验医学》2022年第3期281-287,共7页Laboratory Medicine

基　　金：上海市普陀区重点专科(耳鼻咽喉科)资助项目(2016zk105)。

摘　　要：目的探讨长链非编码RNA(lncRNA)SNHG5对人类下咽癌细胞株FaDu转录组学的影响。方法构建rLV-hSNHG5-ZsGreen-Puro慢病毒和rLV-ZsGreen-Puro对照慢病毒,将感染rLV-hSNHG5-ZsGreen-Puro慢病毒的FaDu细胞作为rLV-SNHG5组,将感染rLV-ZsGreen-Puro对照慢病毒的FaDu细胞作为rLV组。筛选差异基因集并进行基因转录能力、基因本体(GO)功能、京都基因与基因组数据库(KEGG)信号通路、Reactome通路、疾病本体(DO)功能分析。结果rLV-SNHG5组SNHG5相对表达量显著高于rLV组(P<0.01)。基因差异分析共筛选出1070个差异表达的基因,其中表达上调537个、表达下调533个。表达上调居前10位基因分别为LCP1、NT5E、CTGF、AXL、AKAP12、PXDN、THBS1、4-Mar、TMEM200A和RIPOR3,表达下调居前10位基因分别为PKP1、SERPINB13、KLK10、AC011473.4、NOTCH3、CA2、EGLN3、SLC6A8、KRT4和VAV3。功能分析结果显示,SNHG5改变了FaDu细胞部分基因的转录能力、参与调控GO功能、KEGG信号通路、Reactome通路和DO功能。结论SNHG5对FaDu细胞的转录组学有一定的影响。Objective To investigate the effect of long non-coding RNA(lncRNA)small nucleolar RNA host gene 5(SNHG5)on the transcriptome of human hypopharyngeal carcinoma cell line FaDu.Methods The rLV-hSNHG5-ZsGreen-Puro lentivirus and rLV-ZsGreen-Puro control lentivirus were constructed.FaDu cells infected with rLV-hSNHG5-ZsGreen-Puro lentivirus were served as rLV-SNHG5 group,and those infected with rLV-ZsGreen-Puro control lentivirus were served as rLV group.Differential gene sets were screened,and the gene transcriptional ability,Gene Ontology(GO)function,Kyoto Encyclopedia of Genes and Genomes(KEGG)signaling pathway,Reactome pathway and Disease Ontology(DO)function were analyzed.Results The relative expression of SNHG5 in rLV-SNHG5 group was higher than that in rLV group(P<0.01).A total of 1070 differentially expressed genes were screened by gene differential analysis,of which 537 were up-regulated,and 533 were downregulated.The top 10 up-regulated genes with the most significant difference were LCP1,NT5E,CTGF,AXL,AKAP12,PXDN,THBS1,4-Mar,TMEM200A and RIPOR3,and the top 10 down-regulated genes were PKP1,SERPINB13,KLK10,AC011473.4,NOTCH3,CA2,EGLN3,SLC6A8,KRT4 and VAV3.Functional analysis showed that SNHG5 changed the gene transcriptional ability of some genes in FaDu cells and participated in the regulation of GO function,KEGG signaling pathway,Reactome pathway and DO function.Conclusions SNHG5 has a certain effect on the transcriptome of FaDu cells.

关 键 词：小核仁RNA宿主基因5 长链非编码RNA 转录组学 下咽癌 

分 类 号：R393[医药卫生—基础医学]