Bis-cyclometalated Ir(Ⅲ)Complex-Based Electrogenerated Chemiluminescence Sensor Array for Discriminating Three Biothiols  

作　　者：Xiao-Ru Liu Man-Ping Qian Hong-Lan Qi Cheng-Xiao Zhang 

机构地区：[1]Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province,School of Chemistry and Chemical Engineering,Shaanxi Normal University,Xi’an 710062,People’s Republic of China

出　　处：《Journal of Analysis and Testing》2020年第2期114-121,共8页分析检测（英文）

基　　金：the National Natural Science Foundation of China(Nos.21775097,21775098);the Fundamental Research Funds for the Central Universities(GK201801006)。

摘　　要：A simple and sensitive electrogenerated chemiluminescence(ECL)sensor array was developed for the discrimination of three biothiols,including homocysteine(Hcy),cysteine(Cys)and glutathione(GSH)using two bis-cyclometalated Ir(Ⅲ)complexes as ECL probes.Two aldehyde groups bearing bis-cyclometalated Ir(Ⅲ)complexes were selected as ECL probes,including[(bt)2Ir(phen-CHO)]PF_(6)(bt=2-phenylbenzothiazole,phen-CHO=1,10-phenanthroline-5-carboxaldehyde,probe 1)and[(ppy)_(2)Ir(phen-CHO)]PF_(6)(ppy=2-phenylpyridine,probe 2).A“signal on”ECL method was proposed for biothiols assay based on the increase in ECL intensity of two ECL probes by biothiols.Three biothiols can be detected with detection limits of 0.8,0.7 and 0.8μM by probe 1 and 0.3,0.4 and 0.5μM by probe 2 for Cys,Hcy and GSH,respectively.Two-element sensor array was assembled for the detection of three biothiols due to the different enhancement effects of biothiols on the ECL intensity of two ECL probes.The ECL sensor array combining with the principal component analysis was applied to discriminate three biothiols.This study demonstrates that the ECL sensor array using dual ECL reagents provides a promis-ing way for the discrimination of multiple biothiols with good sensitivity and simpleness.

关 键 词：ECL Sensor array Biothiols Ir(Ⅲ)complex Principal component analysis 

分 类 号：O657.3[理学—分析化学]