Electrochemiluminescence Sensor for Cancer Cell Detection Based on H_(2)O_(2)-Triggered Stimulus Response System  

作　　者：Yu Chen Zhonghui Chen Lishan Fang Aibin Weng Fang Luo Longhua Guo Bin Qiu Zhenyu Lin 

机构地区：[1]The Affiliated Hospital of Putian University,Putian University,Putian 351100,Fujian,China [2]Ministry of Education Key Laboratory for Analytical Science of Food Safety and Biology,Fujian Provincial Key Laboratory of Analysis and Detection for Food Safety,Department of Chemistry,Fuzhou University,Fuzhou 350116,Fujian,China

出　　处：《Journal of Analysis and Testing》2020年第2期128-135,共8页分析检测（英文）

基　　金：supported by the National Science Foundation of China(Nos.21675028 and 21775026);the Program for Changjiang Scholars and Innovative Research Team in University(No.IRT15R11);the National Science Foundation of Fujian Province(No.2018J05018);the STS Key Project of Fujian Province(No.2017T3007);Provincial Health Technology Project of Fujian Province(No.2019-ZQN-93).

摘　　要：A H_(2)O_(2)-triggered stimulus response electrochemiluminescence(ECL)sensor for sensitive detection of cancer cells using mesoporous silica nanoparticles(MSNs)has been proposed.ECL signal-generating molecules(Ru(phen)32+)were encap-sulated into phenylboronic acid group-functionalized MSNs(PBA-MSNs)porous and capped by polyhydroxy functioned Au nanoparticles(AuNPs)through the interaction of carbohydrate-boronic acid first.Brunauer-Emmett-Teller(BET)and transmission electron microscopy(TEM)were applied to characterize the materials.The proposed controlled release sensing platform shows approximately no leakage from the mesoporrs of MSNs after a long time of storage.Cancer cells are initially incubated with the functionalized MSNs and then treated with ascorbic acid to endogenously produce H_(2)O_(2).Arylboronic esters in the MSNs surface can be oxidized by the produced H_(2)O_(2),causing the releasing of the molecule from MSNs and increased ECL signal.This technique displayed an excellent measurement for the breast cancer cells’sensitive diagnosis with a detection limit of 208 cells/mL.The phenomenon suggests that this sensing platform may be potentially applied for breast cancer sensitive detection in the future.

关 键 词：ELECTROCHEMILUMINESCENCE Stimulus response sensing Hydrogen peroxide Cancer cell detection 

分 类 号：O657.1[理学—分析化学]