Towards a Versatile Photoreactive Platform for Biosensing Applications  

作　　者：Kamal Elouarzaki Oana Hosu Karine Gorgy Cecilia Cristea Robert Sandulescu Robert SMarks Serge Cosnier 

机构地区：[1]Cambridge CARES,CREATE Tower1 CREATE Way,Singapore 138602,Singapore [2]School of Chemical and Biomedical Engineering,Nanyang Technological University,62 Nanyang Drive,Singapore 637459,Singapore [3]De´partement de Chimie Mole´culaire,Univ.Grenoble Alpes,CNRS,DCM UMR-5250,38000 Grenoble,France [4]Department of Analytical Chemistry,Faculty of Pharmacy,Iuliu Hat¸ieganu,University of Medicine and Pharmacy,400349 Cluj-Napoca,Romania [5]Department of Biotechnology Engineering,Ben-Gurion University of the Negev,84105 Beer-Sheva,Israel

出　　处：《Journal of Analysis and Testing》2017年第2期77-82,共6页分析检测（英文）

基　　金：the Labex ARCANE(ANR-11-LABX-0003-01);Grenoble Institute Carnot POLYNAT(ANR-16-CARN-0025-01);platform Chimie NanoBio ICMG FR 2607(PCN-ICMG).Besides,the authors are grateful for financial support from PHC Braˆncus¸i program 2015–2016 number 32602 QD,the Romanian National Authority for Scientific Research,CNCS—UEFISCDI,Project Number PNII-RU-TE-2014-4-0460 and UMF regarding the internal Grant Number 7690/56/15.04.2016.

摘　　要：An original interfacial hybrid cross-linker molecule combined electrochemical and photochemical properties by substitution of two functional groups,pyrene,and diazirine.The first group enables anchoring by strong p-stacking interaction or by electropolymerization onto multi-walled carbon nanotubes(MWCNT).The other photoreactive group allows the covalent link with biomolecules under long-wavelength UV illumination.Diazirine was chosen due to its ability to photogenerate high reactive radicals under UV irradiation.The photoreactivity of these immobilized nanostructured conductive surfaces was tested towards the covalent attachment of tyrosinase which is well known to oxidize a large range of phenolic compounds and its yield and availability was evaluated by amperometric measurements of catechol by using molecular dioxygen.The architecture exhibiting the best analytical characteristics obtained for catechol was then chosen to detect dopamine.

关 键 词：Photoimmobilization Carbon nanotubes DIAZIRINE TYROSINASE 

分 类 号：TB3[一般工业技术—材料科学与工程]