Quantification of Total Glucosinolates and Isothiocyanates for Common Brassicaceous Vegetables Consumed in the US Market Using Cyclocondensation and Thiocyanate Ion Measurement Methods  

作　　者：Jianghao Sun Pei Chen 

机构地区：[1]Agricultural Research Service,Beltsville Human Nutrition Research Center,Methods and Application of Food Composition Laboratory,US Department of Agriculture,Beltsville,MD 20705,USA

出　　处：《Journal of Analysis and Testing》2019年第4期313-321,共9页分析检测（英文）

基　　金：This research is supported by the Agricultural Research Service of the US Department of Agriculture,an Interagency Agreement with the Office of Dietary Supplements at the National Institutes of Health.

摘　　要：Glucosinolates are a group of important sulfur and nitrogen containing plant secondary metabolites found in Brassicaceous vegetables that are associated with many health benefits.There is a growing interest in assessing dietary glucosinolates and their degradation products’isothiocyanates exposure to humans.Determination of glucosinolates in plants is a very challenging task.In this study,the total glucosinolates from 22 commonly consumed Brassicaceous vegetables in the US market were determined by employing 1,2-benzenedithiol cyclocondensation for the determination of isothiocyanates(ITCs)from non-indole glucosinolates and a colorimetric method for the determination of thiocyanate ion(SCN^(−))from indole glucosi-nolates.The total glucosinolates were determined from the sum of these two methods.In the 22 investigated vegetables,the highest level of total ITCs was found in horse radish(5189.0±544.8 nmol/g dry weight)and the highest level of total SCN^(−)level was found in brussel sprouts(1294.0±141.6 nmol/g dry weight).The total glucosinolates ranged from 18.6±3.2 to 2271.2±215.3 nmol/g dry weight.The total glucosinolate and ITC data are necessary for the USDA glucosinolates database and important for establishing the relationship of glucosinolates to human nutrition.

关 键 词：GLUCOSINOLATE ISOTHIOCYANATE Thiocyanate ion Brassicaceous vegetables 

分 类 号：F42[经济管理—产业经济]