生防菌HY-19的鉴定及其抑菌物质研究  

Study on Identification of Biocontrol Strain HY-19 and Its Antibacterial Substances

作　　者：路兆军 苗杰 刘瑞珍 马旭升 赵莹 王淑惠 李保进 LU Zhao-jun;MIAO Jie;LIU Rui-zhen(Yantai Forest Resources Monitoring and Protection Service Center,Yantai,Shandong 264000)

机构地区：[1]烟台市森林资源监测保护服务中心,山东烟台264000

出　　处：《安徽农业科学》2022年第8期90-93,共4页Journal of Anhui Agricultural Sciences

基　　金：烟台市科技计划项目(2019MSGY114)。

摘　　要：[目的]明确HY-19的抑菌物质特性及其分类地位。[方法]以苹果褐斑病等为靶标采用平板对峙法分析其拮抗谱,对HY-19进行形态观察、生理生化测定和分子鉴定及其抑菌物质特性研究。[结果]菌株HY-19拮抗活性强且抑菌谱广,综合形态、生理生化及系统发育树分析,确定HY-19为短短芽孢杆菌(Brevibacillus brevis)。HY-19无菌滤液对病原指示菌有拮抗活性,且酸碱稳定性、蛋白酶、抗紫外辐射表现良好。HY-19含有丰原素等4种抑菌代谢产物合成基因,表明该菌株可能产生抗菌肽类物质,从而达到抑菌效果。[结论]该研究为苹果病原真菌的生防制剂开发与利用提供科学依据。[Objective]In order to confirm the taxonomic position of HY-19 and its antibacterial substance characteristics.[Method]The inhibitory spectrum of HY-19 targeting to apple pathogenic fungi such as Apple Marssonina Leaf Blotch was analyzed by flat-stand method,then the morphological observation,physiological and biochemical determination,molecular identification and antibacterial properties of HY-19 were studied.[Result]The results showed that HY-19 had strong antagonistic activity and broad spectrum of inhibition.HY-19 was identified as Bacillus brevis through morphological,physiological and biochemical and phylogenetic tree analysis.The fermentation filtrate of HY-19 had antagonistic activity against pathogen indicator which showed good acid-base stability,proteolysis-resistant and UV-resistant.HY-19 contains four kinds of antimicrobial metabolite genes such as fengycin which suggested that the strain may produce antimicrobial peptides to achieve antibacterial effect.[Conclusion]This study will lay a foundation for the development and utilization of biocontrol agents against apple pathogenic fungi.

关 键 词：短短芽孢杆菌 生物防治 苹果病原真菌 抑菌物质 

分 类 号：S432.9[农业科学—植物病理学]