草本组方对γ射线全身照射小鼠的保护作用  

Protection against γ ray-induced damage using herbal formulas

作　　者：付海英[1] 吴国忠[1] FU Haiying;WU Guozhong(Shanghai Institute of Applied Physics,Chinese Academy of Sciences,Shanghai 201800,China)

机构地区：[1]中国科学院上海应用物理研究所,上海201800

出　　处：《辐射研究与辐射工艺学报》2022年第2期35-41,共7页Journal of Radiation Research and Radiation Processing

摘　　要：观察天然草本当归、白芍、肉苁蓉、槐米、黄芪等提取物的混合物(简称草本组方)对γ射线全身照射小鼠的防护及修复作用。将56只成年C57BL/6J小鼠随机分为4组:辐射空白组(Blank)、对照组(Hemo-1#,韩国Hemo Him产品)和两组不同配方给药组(Exp-2#、Exp-3#),每组雌雄性小鼠各7只。给药3 d后,小鼠全身60Coγ射线一次性照射4 Gy,照射后继续连续灌胃21 d,比较小鼠体重及外周血白细胞、红细胞、血小板、淋巴细胞计数等指标变化。结果显示:给药喂养的小鼠(Hemo-1#、Exp-2#、Exp-3#),照射后第1天白细胞和淋巴细胞计数较空白组小鼠下降幅度降低;照射后第7天,小鼠的白细胞、红细胞、血小板、淋巴细胞均缓慢恢复。这表明草本组方能促进照射后小鼠造血功能的修复,推测其保护作用机制与各提取物增强免疫和抗氧化功能有关。Two herbal formulas were prepared by combining extracts from Angelica sinensis, Cistanche deserticola, Paeonia lactiflora pall, Fructus sophorae, and Astragalus and assessed for radioprotective efficacy.Fifty-six healthy C57BL/6J mice were γ irradiated at a dose of 4 Gy to establish a radiation injury model. These mice were randomly divided into four groups with equal numbers of males and females: a radiated vehicle control group,a commercial radioprotective extract control group(Hemo-1#), and two experimental groups(Exp-2# and Exp-3#).The vehicle control group was administered an equal volume of distilled water. The other groups were administered different herbal formulas. Body weight and conventional indicators of peripheral blood homeostasis were recorded before and after irradiation. On the first day after irradiation, mice fed herbal extracts experienced a smaller decrease in leukocyte and lymphocyte counts than untreated mice. At 7 days after irradiation, mouse leukocyte, erythrocyte,platelet, and lymphocyte counts displayed slow recovery. The two groups receiving different herbal formulas showed a similar radioprotective effect on body weight and peripheral blood test results than the Hemo-1# group. Our experimental herbal formulas showed radioprotective effects against γ ray-induced damage.

关 键 词：草本组方 电离辐射损伤 辐射保护 造血功能 小鼠 

分 类 号：TL71[核科学技术—辐射防护及环境保护] Q691.9[生物学—生物物理学]