耐辐射奇球菌对铀的富集性能及其对pH动态调节研究  

Dynamic uranium enrichment and pH regulation in uranium solution by Deinococcus radiodurans

作　　者：陈露瑶 严习 程聪慧 郭可欣 谢靖茜 李珊珊 肖方竹 CHEN Luyao;YAN Xi;CHENG Conghui;GUO Kexin;XIE Jingxi;LI Shanshan;XIAO Fangzhu(School of Public Health,Hengyang Medical College University of South China,Hengyang 421001,China)

机构地区：[1]南华大学衡阳医学院公共卫生学院,衡阳421001

出　　处：《辐射研究与辐射工艺学报》2022年第2期88-96,共9页Journal of Radiation Research and Radiation Processing

基　　金：湖南省自然科学基金项目(2020JJ6050、2020JJ4077)资助。

摘　　要：探索耐辐射奇球菌在不同时间、不同菌剂量、不同初始铀浓度、不同溶液pH下对铀的富集条件以及对铀溶液的pH调节;通过红外光谱和扫描电镜表征其富集机理。结果表明:富集铀的最佳富集时间为60 min、最佳菌剂量为10 mL(当OD_(600)为1时的菌液量)、最佳铀初始质量浓度为30 mg/L、最佳pH为5.0;在不同pH的铀溶液中富集后,溶液pH都略有上升;红外光谱仪和扫描电镜结果显示,耐辐射奇球菌富集前后菌体的多个活性官能团和形貌都发生了变化。耐辐射奇球菌对低浓度铀溶液的富集性能较好;对铀溶液pH有调节作用,使富集后的铀溶液pH上升。Uranium enrichment by Deinococcus radiodurans under different times,bacterial doses,initial uranium concentrations,solution pH values,and pH-adjusted uranium solutions was explored,and the enrichment mechanism was elucidated by Fourier transmission infrared spectroscopy(FTIR)and scanning electron microscopy(SEM).The optimal conditions for adsorption included an enrichment time of 60 min,bacterial dose(i.e.,the volume of bacterial liquid corresponding to an OD_(600) of 1)of 10 mL,initial uranium concentration of 30 mg/L,and solution pH of 5.0.The solution pH increased slightly after enrichment with uranium solutions of different pH.FTIR and SEM analyses revealed several changes in active functional groups and bacterial characteristics before and after enrichment.Deinococcus radiodurans has good enrichment performance for low uranium solution,and it regulated the pH of the uranium solution and increased the pH of the adsorbed uranium solution.

关 键 词：耐辐射奇球菌 铀 富集 调节pH 

分 类 号：R123[医药卫生—环境卫生学] TL94[医药卫生—公共卫生与预防医学]