Detachment-Independent Cationic-Dipeptide Beacons:Reduced False-Negative Signal and Accelerated Fluorescent Lighting in Cell Imaging  

作　　者：Guang-Wei Fu Guo-Yan Luo Xiao-Xuan Li Zhen Zou Yan-Li Lei Zhi-He Qing Jue-Wen Liu Rong-Hua Yang 

机构地区：[1]Hunan Provincial Key Laboratory of Cytochemistry,School of Chemistry and Chemical Engineering,Changsha University of Science and Technology,Changsha 410114,China [2]College of Chemistry and Chemical Engineering,Hunan Normal University,Changsha 410081,China [3]Department of Chemistry,Waterloo Institute for Nanotechnology,University of Waterloo,Waterloo,ON N2L 3G1,Canada

出　　处：《Journal of Analysis and Testing》2022年第1期19-26,共8页分析检测（英文）

基　　金：supported in part by the financial support through the National Natural Science Foundation of China(22074008,91853104,21735001,21705010);the Natural Science Foundation of Hunan Province(2019JJ30025).

摘　　要：Nanocarriers have been developed to deliver DNA probes into cells for imaging analysis and improving their nuclease resist-ance.However,we found obvious lowered sensitivity and even false-negative results in the traditional DNA detachment-dependent fluorescent lighting mechanism.Here,we developed a detachment-independent fluorescent lighting mechanism integrated with cationic dipeptide nanoparticles(CDNs).CDNs displayed little quenching effect on fluorophores labeled on probes and improved the nuclease resistance of probes.In contrast to the traditional beacon,the fluorescence lighting was significantly accelerated without the need of desorption of the recognition products from the nanosurface,avoiding false-negative results from non-specific conformational adsorption.This work will open up new thoughts to improve the sensing performance of DNA probes in cell imaging,such as speed and sensitivity.

关 键 词：Cationic dipeptide nanoparticles DNA probe False-negative signal Fluorescence Cell imaging 

分 类 号：O657.3[理学—分析化学] Q2-33[理学—化学]