A Thermophilic G-Quadruplex DNA/N-methylmesoporphyrin IX Sensor for Accurately Detecting Single Nucleotide Polymorphism  

作　　者：Yu Yan Dan Zhao Qiang Zhang Yang-Yang Chang Meng Liu 

机构地区：[1]School of Environmental Science and Technology,Key Laboratory of Industrial Ecology and Environmental Engineering(Ministry of Education),Dalian University of Technology,Dalian 116024,China [2]School of Bioengineering,Dalian University of Technology,Dalian 116024,China

出　　处：《Journal of Analysis and Testing》2022年第1期44-50,共7页分析检测（英文）

基　　金：financially supported by the National Natural Science Foundation of China (21922601, 51808096);Liaoning Revitalization Talents Program (XLYC1807080);the Fundamental Research Funds for the Central Universities (DUT20YG123)

摘　　要：We found U2R1,a previously discovered G-rich DNA sequence,could form the G-quadruplex(G4)structure at high tem-perature and maintain high stability.By utilizing its specific binding ability with the N-methylmesoporphyrin IX(NMM,a fluorescent probe),a thermophilic signal transduction unit was proposed,that was the NMM-U2R1 complex.Current methods for single nucleotide polymorphism(SNP)detection relying on the room-temperature rolling circle amplification system often suffered from poor accuracy,since the low temperature lowers the sensitivity for identifying the base mismatches.In this work,we combined the thermal stable signal transduction unit with the isothermal amplification reaction to develop a thermophilic fluorescent assay.High temperature could ensure the accuracy of base pairing.Based on this,this fluorescent assay has been successfully applied for the identification of one-or two-mismatched base DNA or microRNA 21.And it is expected to be generally applicable to identify SNPs in many other sequences.Furthermore,this work will open new opportunities for development of the thermally stable G4 DNA in biosensor.

关 键 词：THERMOPHILIC G-quadruplex DNA Isothermal amplification Single nucleotide polymorphism 

分 类 号：O657.3[理学—分析化学] Q503[理学—化学]