Pyrophosphate-Mediated On-Off-On Oxidase-Like Activity Switching of Nanosized MnFe_(2)O_(4) for Alkaline Phosphatase Sensing  

作　　者：Xiangheng Niu Kun Ye Zhibo Li Hongli Zhao Linjie Wang Jianming Pan Hongwei Song Minbo Lan 

机构地区：[1]Institute of Green Chemistry and Chemical Technology,School of Chemistry and Chemical Engineering,Jiangsu University,Zhenjiang 212013,China [2]Shanghai Key Laboratory of Functional Materials Chemistry,School of Chemistry and Molecular Engineering,East China University of Science and Technology,Shanghai 200237,China [3]School of Environmental and Chemical Engineering,Jiangsu University of Science and Technology,Zhenjiang 212003,China

出　　处：《Journal of Analysis and Testing》2019年第3期228-237,共10页分析检测（英文）

基　　金：This study was supported by the National Natural Science Foundation of China(21605061 and 31601549);the Natural Science Foundation of Jiangsu Province(BK20160489);the Open Fund from the Shanghai Key Laboratory of Functional Materials Chemistry(SKLFMC201601);the Cultivation Project for Excellent Young Teachers in Jiangsu University.

摘　　要：Developing reliable and facile approaches for alkaline phosphatase(ALP)sensing is important due to its role as a clinical biomarker for many diseases.In this study,we proposed a new and convenient colorimetric assay based on the pyrophosphate(PPi)-mediated oxidase-mimicking activity switching of nanosized MnFe_(2)O_(4) for the detection of ALP.The synthesized MnFe_(2)O_(4) exhibited high oxidase-like activity to catalyze the oxidation of colorless 3,3′,5,5′-tetramethylbenzidine(TMB)to its blue product TMBox in the presence of dissolved O2,leading to a color reaction rapidly and remarkably;PPi could significantly inhibit the activity of the MnFe_(2)O_(4) nanozyme via the strong interaction between PPi and the Fe(III)species in MnFe_(2)O_(4),resulting in the suppression of the TMB color reaction;when ALP was added,it hydrolyzed the PPi substrate to phosphate(Pi)that had no obvious effect on the MnFe_(2)O_(4) activity,and such that the TMB color reaction catalyzed by the nanozyme could be observed again.With the above principle,linear colorimetric determination of ALP in the scope of 0.6-55 U L−1 was achieved,giving the limit of detection down to 0.27 U L−1.Besides,the developed assay could provide selective response toward ALP against other co-existing biological species.Furthermore,reliable detection of ALP in human serum samples was verified by our assay,revealing its great promise as an effective and facile tool for ALP monitoring in clinical practice.

关 键 词：Oxidase-like nanozyme Activity switching PPi ALP Colorimetric analysis 

分 类 号：O62[理学—有机化学]