Porous Ruthenium Selenide Nanoparticle as a Peroxidase Mimic for Glucose Bioassay  

作　　者：Wen Cao Junshu Lin Faheem Muhammad Quan Wang Xiaoyu Wang Zhangping Lou Hui Wei 

机构地区：[1]Department of Biomedical Engineering,College of Engineering and Applied Sciences,Nanjing National Laboratory of Microstructures,Jiangsu Key Laboratory of Artificial Functional Materials,Nanjing University,Nanjing 210093,Jiangsu,China [2]Department of Biomaterials,College of Materials,Xiamen University,Xiamen 361005,Fujian,China [3]State Key Laboratory of Analytical Chemistry for Life Science and State Key Laboratory of Coordination Chemistry,School of Chemistry and Chemical Engineering,Nanjing University,Nanjing 210023,Jiangsu,China

出　　处：《Journal of Analysis and Testing》2019年第3期253-259,共7页分析检测（英文）

基　　金：This work was supported by National Natural Science Foundation of China(21874067 and 21722503);973 Program(2015CB659400);PAPD program,Shuangchuang Program of Jiangsu Province,Open Funds of the State Key Laboratory of Analytical Chemistry for Life Science(SKLACLS1704);Open Funds of the State Key Laboratory of Coordination Chemistry(SKLCC1819);Key Laboratory of Analytical Chemistry for Biology and Medicine(Ministry of Education)(ACBM2019001);Fundamental Research Funds for the Central Universities(021314380145).

摘　　要：Nanozyme is a promising field that offers the substitution for natural enzymes using various nanomaterials.Various nanoma-terials with peroxidase-like activity were investigated.Among them,transition metal chalcogenides were explored as promis-ing nanozymes due to their excellent enzyme-mimicking activities.However,ruthenium selenide has not been studied as a peroxidase mimic because of the difficulty for synthesis.Herein,we prepared ruthenium selenide nanomaterial with ordered mesoporous structure(P-RuSe_(2))employing KIT-6 silica as the template.The composition and structure of P-RuSe_(2) were fully characterized.Further,its peroxidase-like activity was investigated.P-RuSe_(2) possessed excellent peroxidase-mimicking activ-ity,which catalyzed the oxidation of peroxidase substrates,including 3,3′,5,5′-tetramethylbenzidine,o-phenylenediamine,and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)in the presence of H_(2)O_(2).Moreover,P-RuSe_(2) exhibited higher peroxidase-like activity when compared with several representative nanozymes as well as bulk RuSe_(2).To demonstrate its potential applications,the colorimetric detection systems for H_(2)O_(2) and glucose were successfully constructed based on P-RuSe_(2) nanozyme.

关 键 词：Nanozymes Peroxidase mimics Artificial enzymes Ruthenium selenide BIONANOTECHNOLOGY Glucose bioassay 

分 类 号：O64[理学—物理化学]