458份小麦品种(系)抗病性状功能基因的KASP标记检测  

KASP Marker Assays for Functional Genes of Disease Resistance Traits in 458 Wheat Cultivars(Lines)

作　　者：王重[1,2] 张宏芝 高新[1,3] 时佳 王立红[1,2] 李剑峰 樊哲儒 赵奇 张跃强[1,2] WANG Zhong;ZHANG Hongzhi;GAO Xin;SHI Jia;WANG Lihong;LI Jianfeng;FAN Zheru;ZHAO Qi;ZHANG Yueqiang(Research Institute of Nuclear Technology and Biotechnology,Xinjiang Academy of Agricultural Sciences,Urumqi 830091,China;Key Laboratory of Desert-Oasis Crop Physiological Ecology and Cultivation,Ministry of Agriculture and Rural Affairs of the P.R.C.,Urumqi 830091,China;Key Laboratory of Xinjiang Crop Biotechnology,Urumqi 830091,China)

机构地区：[1]新疆农业科学院核技术生物技术研究所,乌鲁木齐830091 [2]农业部荒漠绿洲作物生理生态与耕作重点实验室,乌鲁木齐830091 [3]新疆农作物生物技术重点实验室,乌鲁木齐830091

出　　处：《新疆农业科学》2022年第4期781-786,共6页Xinjiang Agricultural Sciences

基　　金：新疆维吾尔自治区科技支疆项目(2019E0250)。

摘　　要：【目的】研究不同小麦品种(系)抗病性状功能基因的分布状态,分析小麦品种(系)的抗病功能基因,为小麦抗病育种提供理论依据。【方法】利用KASP技术通过1个抗白粉病分子标记(Pm21)、1个抗条锈病分子标记(Yr15)和2个抗叶锈病分子标记(Lr14、Lr68),检测458份小麦品种(系)。【结果】筛选出携带Pm21标记材料2份,Yr15标记材料14份,Lr14a标记材料22份,Lr68标记材料48份。【结论】新疆小麦品种(系)中抗病基因的分布频率较低,约为5%。筛选出的86份材料可以作为新疆小麦抗病育种的亲本材料加以应用。【Objective】This project aims to study the distribution of functional genes for disease resistance of different wheat varieties(lines)and clarify the in the hope of providing theoretical basis for wheat disease resistance breeding.【Method】458 wheat varieties(lines)were detected by KASP through one powdery mildew resistance molecular marker(Pm21),one stripe rust resistance molecular marker(Yr15)and two leaf rust resistance molecular markers(Lr14a and Lr68).【Result】There were 2 Pm21 carried lines,14 Yr15 carried lines,22 Lr14a carried lines and 48 Lr68 carried lines in 458 wheat cultivars(lines).【Conclusion】The distribution frequency of resistance genes in Xinjiang wheat varieties(lines)was about 5%and the 86 lines selected can be used as the parents for resistance breeding of wheat in Xinjiang.

关 键 词：小麦 抗病基因 KASP 标记检测 

分 类 号：S512[农业科学—作物学]