基于计算机模拟的人工盐碱地盐分配方及其应用  

A Formula for the Salt of Artificial Saline-alkali Soil and Its Application Based on Computer Simulation

作　　者：王伟 于亮[1] 王志 王伟伟[1] 钮力亚[1] 陆莉[1] 王奉芝[1] 张景俭 WANG Wei;YU Liang;WANG Zhi;WANG Weiwei;NIU Liya;LU Li;WANG Fengzhi;ZHANG Jingjian(Cangzhou Academy of Agriculture and Forestry Sciences/Hebei Key Laboratory of Crop Salt-alkali Tolerance Evaluation and Genetic Improvement,Cangzhou,Hebei 061001 China)

机构地区：[1]沧州市农林科学院/河北省农作物耐盐碱评价与遗传改良重点实验室,河北沧州061001

出　　处：《作物研究》2022年第2期132-137,共6页Crop Research

基　　金：河北省“三三三人才工程”项目(A202103013);“智汇沧州”人才立市重点项目(023);沧州市农林科学院博士基金(D0001);国家现代农业产业技术体系(CARS-03)。

摘　　要：为了构建快速鉴定小麦盐碱耐性的方法,基于河北省滨海盐碱地主要盐分离子的检测数据,利用重抽样自举法进行各盐分离子总体的参数估计,再根据土壤中阴阳离子电荷平衡组配化学试剂,筛选贴近自然环境条件下滨海盐碱地实际盐分的化学试剂配方,并通过小麦芽期盐碱耐性鉴定试验对配方的有效性进行验证。结果表明:模拟中度盐碱地盐分配方的盐分水平适用于小麦芽期盐碱耐性鉴定,该配方中各组分及其含量分别为氯化钠1.274 6 g/L、氯化钾0.015 7 g/L、氯化镁0.361 9 g/L、氯化钙0.263 1 g/L、碳酸氢钠0.330 2 g/L、硫酸钠0.838 0 g/L、硅酸钠0.079 6 g/L。应用此配方,采用隶属函数法、主成分分析法和聚类分析法开展小麦芽期盐碱耐性鉴定与评价,鉴定结果与小麦田间实际表现吻合。本研究结果可为室内准确、快速、高通量的植物耐盐碱鉴定方法的构建提供参考。In order to establish a method for rapid identification of salt-alkali tolerance of wheat, based on the detection data of main salt ions in coastal saline-alkali land of Hebei Province, the parameters of each salt ion population were estimated by re-sampling bootstrap method. Then, according to the charge balance of anion and cation in soil, the chemical reagent formula close to the actual salinity of coastal saline-alkali land under natural environment conditions was screened. The effectiveness of the formula was verified by the identification test of saline-alkali tolerance in wheat sprout stage. The results showed that the salt level of the simulated salt formula in moderate saline-alkali soil was suitable for the identification of saline-alkali tolerance of wheat at budding stage. The components and their contents in the formula were sodium chloride 1.274 6 g/L,potassium chloride 0.015 7 g/L,magnesium chloride 0.361 9 g/L,calcium chloride 0.263 1 g/L,sodium bicarbonate 0.330 2 g/L,sodium sulfate 0.838 0 g/L,sodium silicate 0.079 6 g/L. Using this formula, subordinate function values analysis, principal component analysis and cluster analysis were used to identify and evaluate the saline-alkali tolerance of wheat at budding stage, and the results were consistent with the actual performance of wheat in the field. The results of this study can provide a reference for accurate, rapid and high-throughput identification of plant saline-alkali tolerance in laboratory.

关 键 词：植物 盐碱耐性 鉴定 配方 重抽样自举法 

分 类 号：Q945.78[生物学—植物学]