脂肪酸结合蛋白4在卵泡液局部的作用及其机制探讨  

A STUDY OF THE ROLE OF FATTY ACID-BINDING PROTEIN 4 IN FOLLICULAR FLUID AND ITS MECHANISM

作　　者：夏晨 刘姝含[1] 耿颖春 汤秀明[1] 王琳[1] 刘建娥 刘建新[1] XIA Chen;LIU Shuhan;GENG Yingchun;TANG Xiuming;WANG Lin;LIU Jian′e;LIU Jianxin(Department of Reproductive Medicine, The Affiliated Hospital of Qingdao University, Qingdao 266003, China)

机构地区：[1]青岛大学附属医院生殖医学科,山东青岛266003

出　　处：《精准医学杂志》2022年第2期110-114,共5页Journal of Precision Medicine

基　　金：青岛市民生科技计划项目(16-6-2-21-nsh)。

摘　　要：目的研究卵泡液中脂肪酸结合蛋白4(FABP4)与卵泡液局部炎症及脂代谢的关系,并分析其对卵母细胞功能的潜在影响。方法本研究纳入2020年6月-2021年1月在青岛大学附属医院生殖医学科因单纯输卵管和(或)男性因素接受体外受精/卵胞质内单精子注射-胚胎移植受试者122例。根据受试者卵泡液中FABP4浓度的百分位数分为3个浓度组:A组(FABP4浓度≤24.56μg/L,P_(0)~P_(25)),B组(24.56μg/L<FABP4浓度≤48.36μg/L,P_(26)~P_(75)),C组(FABP4浓度>48.36μg/L,P_(76)~P_(100))。比较3组受试者卵泡液中单核细胞趋化蛋白-1(MCP-1)、白细胞介素-6(IL-6)、氧化低密度脂蛋白(ox-LDL)、总胆固醇(TC)、低密度脂蛋白胆固醇(LDL-C)及甘油三酯(TG)水平的差异。采用多重线性回归分析优质胚胎率的影响因素。结果A、B和C组卵泡液中MCP-1、IL-6、ox-LDL水平随FABP4浓度增高而增高,组间比较差异有显著性(H=11.371~54.114,P<0.01);两两比较结果显示,A组MCP-1、ox-LDL水平均显著低于B、C组(H=-65.017~-19.447,P<0.01);A组IL-6水平显著低于C组(H=-30.633,P<0.01);B组MCP-1、ox-LDL水平显著低于C组(H=-36.337、-44.331,P<0.01);3组受试者卵泡液中LDL-C、TC和TG水平比较差异无显著性(P>0.05)。多重线性回归显示,卵泡液中FABP4是优质胚胎率的危险因素(β=-0.520,95%CI=-0.981~-0.059,P<0.05),且该作用独立于卵泡液IL-6、ox-LDL、促性腺激素使用总量和体质量指数。结论卵泡液中FABP4可能通过与MCP-1、IL-6和ox-LDL的相互作用参与局部炎症和氧化应激,并可能影响卵母细胞质量。Objective To investigate the relationship between fatty acid-binding protein 4(FABP4)with local inflammation and lipid metabolism in follicular fluid(FF),as well as the potential influence of FABP4 on oocyte function.Methods A total of 122 patients who underwent in vitro fertilization/intracytoplasmic sperm injection-embryo transfer(IVF/ICSI-ET)due to simple fallopian tube and/or partner problems in the Department of Reproductive Medicine,The Affiliated Hospital of Qingdao University from June 2020 to January 2021 were enrolled in this cross-sectional study.According to the FF FABP4 level,the patients were divided into group A(FABP4 level≤24.56μg/L,P_(0)-P_(25)),group B(24.56μg/L<FABP4 level≤48.36μg/L,P_(26)-P_(75)),and group C(FABP4 level>48.36μg/L,P_(76)-P_(100)).The three groups were compared in terms of the levels of FF monocyte chemoattractant protein-1(MCP-1),interleukin-6(IL-6),oxidized low-density lipoprotein(ox-LDL),total cholesterol(TC),low-density lipoprotein cholesterol(LDL-C),and triglyceride(TG).Afterwards,multivariate linear regression was used to analyze the influencing factors for high-quality embryo rate.Results The concentrations of FF MCP-1,IL-6,and ox-LDL significantly increased as the FABP4 level increased in groups A,B,and C(H=11.371-54.114,P<0.01).The pairwise comparisons showed that group A had significantly lower levels of FF MCP-1 and ox-LDL compared with group B and C(H=-65.017--19.447,P<0.01)and a significantly lower level of FF IL-6 compared with group C(H=-30.633,P<0.01);group B had significantly lower levels of FF MCP-1 and ox-LDL compared with group C(H=-36.337,-44.331,P<0.01);but there were no significant differences in the concentrations of FF LDL-C,TC,and TG between the three groups(P>0.05).The multivariate li-near regression showed that FF FABP4 was a risk factor for the high-quality embryo rate(β=-0.520,95%CI=-0.981--0.059,P<0.05),which was independent of the FF IL-6 and ox-LDL levels,total dosage of gonadotropin,and body mass index.Conclusion FABP4 may be involved i

关 键 词：脂肪酸结合蛋白4 卵泡液 趋化因子CCL2 白细胞介素6 脂蛋白类 LDL 氧化性应激 炎症 卵母细胞 

分 类 号：R321.1[医药卫生—人体解剖和组织胚胎学] R321-33[医药卫生—基础医学]