晋谷21号成熟胚再生体系的优化  

Optimization of Regeneration System of Mature Embryo of Jingu 21

作　　者：王玉玲 张世芳 尹艺臻[1] 马春英 王育选[1] 刘清丽[2] 赵娟[1] WANG Yuling;ZHANG Shifang;YIN Yizhen;MA Chunying;WANG Yuxuan;LIU Qingli;ZHAO Juan(College of Agriculture,Shanxi Agricultural University,Taigu 030801,China;Liaoning Agricultural Technical College,Yingkou 115009,China)

机构地区：[1]山西农业大学农学院,山西太谷030801 [2]辽宁农业职业技术学院,辽宁营口115009

出　　处：《山西农业科学》2022年第4期469-477,共9页Journal of Shanxi Agricultural Sciences

基　　金：财政部和农业农村部:国家现代农业产业技术体系(CARS-06-13.5-A28);山西农业大学省部共建有机旱作农业国家重点实验室自主研发项目(202003-5);山西农业大学青年拔尖创新人才支持计划(TYIT201406);山西省粮食系统研发项目(202001);山西省重点研发计划项目(201903D221030);山西省回国留学人员科研资助项目(2020-068)。

摘　　要：为了提高谷子成熟胚愈伤组织的诱导率和分化率,建立高效、稳定的再生体系,以优质品种晋谷21号成熟胚为外植体,研究植物激素、蔗糖、硝酸银(Ag NO;)、多效唑(PP_(333))等不同物质对晋谷21号愈伤组织诱导、继代、分化和生根的影响,进而优化了晋谷21号离体再生培养方案,最终确定了晋谷21号各阶段的最适培养基和激素浓度。结果表明,在MS培养基中加入2.0 mg/L 2,4-二氯苯氧乙酸(2,4-D)、0.2 mg/L 6-苄氨基嘌呤(6-BA)、5.0%蔗糖时愈伤组织诱导率最高,可达95.24%,较前期试验筛选出的诱导培养基(2.0 mg/L 2,4-D、0.2 mg/L 6-BA、3.0%蔗糖)提高了2.38百分点,产生的愈伤组织致密,颜色淡黄,状态更佳,有利于进一步的分化;再分化过程中添加1.0 mg/L 6-BA、0.5 mg/L NAA、3.0 mg/L PP_(333)、3.0%蔗糖、14 g/L琼脂时分化率最高,可达28.33%,较前期试验筛选出的分化培养基(1.0 mg/L 6-BA、3.0%蔗糖、5 g/L琼脂)提高了15百分点,且分化出的谷苗健壮;生根阶段最适培养基为1/2 MS培养基,生根健壮,适合移栽。In order to improve the callus induction and differentiation rate of mature embryos of millet and to establish an efficient and stable regeneration system,in this study,mature embryos of a foxtail millet cultivar Jingu 21 with excellent quality was used as explant.The effects of the substances including plant hormones,sucrose,silver nitrate(Ag NO;)and paclobutrazol(PP_(333))on callus induction,subculture,differentiation and rooting of Jingu 21 were studied,and the in vitro regeneration culture scheme of Jingu 21 was optimized,finally,the optimum concentrations of culture mediums and hormones in stages of Jingu 21 were defined.The results showed when 2.0 mg/L of 2,4-dichlorophenoxyacetic acid(2,4-D),0.2 mg/L of 6-benzylaminopurine(6-BA)and5.0%of sucrose were added to MS medium,the callus induction rate was the highest,it reached 95.24%,compared with the induction medium screened out in the previous experiment with the addition of 2.0 mg/L of 2,4-D,0.2 mg/L of 6-BA and 3.0%of sucrose,increased by 2.38 percentage points.Additionally,the callus produced was dense,yellowish and better,which was beneficial to further differentiation.In the process of redifferentiation,when 1.0 mg/L of 6-BA,0.5 mg/L of naphthylacetic acid(NAA),3.0 mg/L of PP_(333),3.0%of sucrose,and 14 g of agar were added,the differentiation rate was the highest,it reached28.33%,compared with the differentiation medium screened in the previous experiment with the addition of 1.0 mg/L of 6-BA,3.0%of sucrose and 5 g/L of agar,increased by 15 percentage points,and the differentiated millet seedlings were strong.The optimum medium for rooting stage was 1/2 MS medium,in which roots were robust and suitable for transplanting.

关 键 词：谷子 成熟胚 愈伤组织 植物激素 附加物 

分 类 号：S515[农业科学—作物学]