鼠疫耶尔森菌显色培养基的筛选试验  

Screening test of Yersinia pestis chromogenic medium

作　　者：谢辉[1] 赵小龙[1] 刘中凯 崔百忠[1] XIE Hui;ZHAO Xiao-long;LIU Zhong-kai;CUI Bai-zhong(Qinghai Institute for Endemic Disease Prevention and Control,Xining,Qinghai 811602,China)

机构地区：[1]青海省地方病预防控制所,青海西宁811602

出　　处：《现代预防医学》2022年第8期1490-1494,共5页Modern Preventive Medicine

基　　金：国家卫生健康委员会鼠疫防治研究重点实验室(青海省地方病预防控制所)(2019PT310004);鼠疫防控及研究重点实验室项目(2021-ZJ-Y15)。

摘　　要：目的 根据鼠疫菌对某些抑菌剂的抵抗力和酶底物显色的方法研制一种鼠疫菌显色培养基,并对其进行检测效果评价,方法 以耶尔森菌选择性培养基(CIN琼脂)为基础添加促进鼠疫菌生长的营养成分以及不同浓度抑菌剂,制备4种鼠疫菌显色培养基,通过单因素实验,筛选出最佳鼠疫菌显色培养基。结果 经单因素筛选出的1%胆盐显色培养基对常见致病菌具有较强的抑制作用,对鼠疫菌具有较好的选择性和特异性,经28℃培养18~24 h鼠疫菌呈玫红色菌落,菌落典型,易于分离,1%胆盐显色培养基鼠疫菌菌落计数与赫氏培养基相比,没有统计学差异。结论 优化的鼠疫菌显色培养基具有显色效果好、特异性强、敏感度高,可提高鼠疫菌的分离率,适用于鼠疫菌分离培养的初筛。Objective To develop a chromogenic medium for Yersinia pestis based on the resistance of Yersinia pestis to certain inhibitors and the color development of enzymatic substrates, and to evaluate its detection effect. Methods Four Yersinia pestis color development media were prepared based on Yersinia pestis selective medium(CIN agar) with nutrients to promote the growth of Yersinia pestis and different concentrations of bacteriostatic agents, and the best Yersinia pestis color development media were screened by single-factor experiments. Results The 1% bile salt chromogenic medium screened by single factor had strong inhibitory effect on common pathogenic bacteria and good selectivity and specificity for Yersinia. Yersinia showed rosy red colonies after incubation at 28℃ for 18-24 h, and the colonies were typical and easy to be separated. Conclusion The optimized chromogenic medium of Yersinia pestis has good chromogenic effect, strong specificity and high sensitivity, which can improve the isolation rate of Yersinia pestis and is suitable for the primary screening of Yersinia pestis isolation culture.

关 键 词：鼠疫耶尔森菌 显色培养基 耶尔森菌选择性培养基 

分 类 号：R378[医药卫生—病原生物学]