妃子笑荔枝LcSAI启动子的克隆及其生物信息学分析  

Cloning and Bioinformatics Analysis of LcSAI Gene Promoter from Feizixiao Litchi

作　　者：董晨[1] 李金枝 郑雪文 王弋[1] 李伟才[1] DONG Chen;LI Jinzhi;ZHENG Xuewen;WANG Yi;LI Weicai(Institute of South Subtropical Corp Research,Chinese Academy of Tropical Agricultural Science/Key Laboratory of Tropical Fruit Biology,Ministry of Agriculture and Rural Affairs,Zhanjiang 524091,China)

机构地区：[1]中国热带农业科学院南亚热带作物研究所/农业农村部热带果树生物学重点实验室,广东湛江524091

出　　处：《广东农业科学》2022年第3期25-30,共6页Guangdong Agricultural Sciences

基　　金：广东省自然科学基金(2018A030307012);国家重点研发计划项目(2019YFD1000900);中央级公益性科研院所基本科研业务专项(1630062021010);国家荔枝龙眼产业技术体系专项(CARS-32-20)。

摘　　要：【目的】酸性转化酶在荔枝糖代谢中具有重要作用,克隆和分析荔枝酸性转化酶基因(LcSAI)启动子,以期为LcSAI调控荔枝糖代谢的功能研究提供理论依据。【方法】以妃子笑荔枝果肉为材料,根据荔枝基因组序列信息,克隆LcSAI上游约1 500 bp的启动子序列,并利用生物信息学工具对启动子序列中的转录起始位点、顺式作用元件及CpG岛进行预测分析。【结果】克隆得到1 514 bp的LcSAI启动子序列,启动子顺式作用元件除了存在大量的启动子核心元件如TATA-box和增强元件CAAT-box外,还存在光响应顺式元件、激素应答元件、厌氧诱导响应元件、MYB结合位点、MYC结合位点及一些未知功能的顺式作用元件。LcSAI启动子序列可能存在3处核心启动子区域,LcSAI启动子区没有预测到符合限定条件的CpG岛。【结论】LcSAI基因启动子与逆境胁迫有关,推测酸性转化酶在荔枝抵御逆境胁迫的生理过程中具有重要作用。【Objective】Acid invertase(SAI) plays an important role in litchi sugar metabolism. The litchi LcSAI promoter is cloned and analyzed in order to provide a theoretical basis for the research on the function of LcSAI in regulating litchi sugar metabolism.【Method】With the pulp of Feizixiao litchi as test material, according to the litchi genome sequence information, the 1 500 bp promoter sequence upstream of LcSAI gene was cloned. At the same time, the transcription start sites, cis-acting elements and CpG islands in the promoter sequences were predicted and analyzed by bioinformatics tools.【Result】A 1 514 bp LcSAI promoter sequence was cloned. In addition to a large number of promoter core elements such as TATA-box and enhancer element CAAT-box, promoter cis-acting elements also had light-responsive cis-elements, hormone response elements, anaerobic inducible response elements, and MYB binding sites, MYC binding sites and some cis-acting elements of unknown function. There may be three core promoter regions in the LcSAI promoter sequence. The LcSAI promoter region did not predict CpG islands that met the defined conditions.【Conclusion】The LcSAI gene promoter is related to adversity stress. It is predicted that LcSAI plays an important role in the physiological process of litchi resisting adversity stress.

关 键 词：妃子笑荔枝 酸性转化酶基因 启动子 克隆 生物信息学 

分 类 号：S667.1[农业科学—果树学]