鱼类细胞培养历史、应用及培养条件研究概述  

An Overview of Research on Culture History,Application and Culture Conditions of Fish Cell

作　　者：毛美琴 蓝桢宇 李进 郭锡燚 黄凤萍 廖元龙 蔡小辉 MAO Meiqin;LAN Zhenyu;LI Jin;GUO Xiyi;HUANG Fengping;LIAO Yuanlong;CAI Xiaohui(College of Marine Sciences,Beibu Gulf University/Guangxi Key Laboratory of Beibu Gulf Marine Biodiversity Conservation,Qinzhou 536011,China)

机构地区：[1]北部湾大学海洋学院/广西北部湾海洋生物多样性养护重点实验室,广西钦州536011

出　　处：《广东农业科学》2022年第3期124-134,共11页Guangdong Agricultural Sciences

基　　金：广西自然科学基金(2018GXNSFAA281208和2019GXNSFBA245062);广西科技开发项目(桂科AB18221112);广西北部湾海洋珍稀物种养护自治区重点实验室项目(2021ZB01)。

摘　　要：细胞培养指的是经过酶、机械或化学的方法将组织离散成单个细胞,模拟动物体内环境进行培养并促进其生长发育的方法。基于鱼类模型的潜在应用,开发了包括体外细胞培养在内的多种技术。20世纪生物技术兴起,体外细胞培养逐渐渗透至各学科应用,如免疫学、药理学、毒理学、遗传及组织再生和移植等。体外培养细胞具有模仿宿主细胞遗传同质性的优点,实验过程中可一定程度上减少体内的反应变异性,且成本低、重复性好、道德约束小。鱼类细胞培养技术发展于20世纪60年代,最早应用于病毒分离,细胞可从不同器官组织中获得。虽然鱼类细胞培养技术研究晚于哺乳动物,但从长远来看对于鱼类细胞的深入研究对于理论研究和实际应用都具有深远意义和广阔开发前景。简要讨论了鱼类细胞培养的历史进程、应用现状以及细胞培养技术,为未来鱼类细胞培养技术研究提供参考。Cell culture refers to the method that tissues are separated into single cells by enzyme, mechanical or chemical methods, and the growth and development of cells are simulated by the animal’s internal environment. Based on the potential applications of the fish model, various tools and techniques, including in vitro cell culture, have been developed.With the rise of biotechnology in the 20th century, in vitro cell culture has gradually been penetrated into various disciplines,such as immunology, pharmacology, toxicology, genetics and tissue regeneration and transplantation. In vitro cultured cells have the advantage of imitating the genetic homogeneity of host cells, and the reaction variability in vivo can be reduced to a certain extent in the experimental process, with low cost, good repeatability, and less moral constraints. Fish cell culture technology was developed in the 1960s and was first applied to virus isolation. Cells can be obtained from different organs and tissues. Although the research of fish cell culture technology is later than that of mammals, in the long run, the indepth study of fish cells has far-reaching significance and broad development prospects for both theoretical research and practical application. This review briefly discusses the history, application and technology of fish cell culture, which provides reference for future research on fish cell culture technology.

关 键 词：细胞培养技术 鱼类细胞 应用现状 发展方向 

分 类 号：S917.4[农业科学—水产科学]