一株腐殖质还原菌的分离鉴定及其对敌草快的厌氧降解特性  

Isolation, and identification of a humus-reducing bacteria and its anaerobic degradation characteristics of diquat

作　　者：阴文芳 成剑波 吴东明[3,4] 武春媛 YIN Wen-fang;CHENG Jian-bo;WU Dong-ming;WU Chun-yuan(Guizhou University,College of Agriculture,Guiyang 550025,China;Guizhou University,Institute of New Rural Development,Guiyang 550025,China;Institute of Environment and Plant Protection,Chinese Academy of Tropical Agricultural Sciences,Haikou 571101,China;National Agricultural Experimental Station for Agricultural Environment,Danzhou 571737,Hainan,China)

机构地区：[1]贵州大学农学院,贵阳550025 [2]贵州大学新农村发展研究院,贵阳550025 [3]中国热带农业科学院环境与植物保护研究所,海口571101 [4]国家农业环境循州观测实验站,海南脩州571737

出　　处：《农药》2022年第4期260-265,共6页Agrochemicals

基　　金：海南省自然科学基金(420QN315);国家自然科学基金(42177402);中国热带农业科学院基本科研业务费专项资金(1630042021013)。

摘　　要：[目的]分离筛选出具有腐殖质还原能力且能厌氧降解敌草快的菌株。[方法]以蒽醌-2,6-二磺酸二钠(AQDS)为唯一电子受体,从河流沉积物中富集分离具有腐殖质还原能力的菌株,通过形态学和生理生化特征,以及16S rRNA基因序列分析对菌株进行鉴定;筛选其电子供受体谱;并研究其厌氧条件下对敌草快的降解特性。[结果]获得1株具有腐殖质还原能力的菌株HN05,鉴定为Kosakonia oryzae HN05。HN05能以蔗糖、葡萄糖、乳糖、乳酸、丙三醇、乙酸、乙醇、甲醇作为电子供体还原AQDS,利用能力依次递减。HN05能以蔗糖作为电子供体还原腐殖质模式物蒽醌-1-磺酸钠(α-AQS)、蒽醌-2-磺酸钠(AQS)、AQDS、蒽醌-1,5-二磺酸钠(1,5-AQDS)。HN05能以蔗糖作为电子供体厌氧降解敌草快,但降解率较小;加入AQDS能极大促进敌草快降解,22 d降解率可达41.93%。[结论]试验首次报道水稻科萨克氏菌HN05具有腐殖质还原以及降解敌草快的能力,丰富了腐殖质还原菌的菌种资源及其功能多样性,可为敌草快污染环境修复提供基础。[Aims] This study aims to isolate and screen out a strain that has the ability to reduce humus and anaerobic degradation diquat. [Methods] Using anthraquinone-2,6-disulfonic acid disodium(AQDS) as the only electron acceptor,the strain with humus reduction ability was enriched and isolated from river sediments. The strains were identified based on morphological, physiological and biochemical characteristics, and 16S r RNA gene sequence analysis;the electron donor and acceptor profiles were screened;and the degradation characteristics of diquat under anaerobic conditions were studied. [Results] Strain HN05 with humus reduction ability was obtained, and it was identified as Kosakonia oryzae HN05. HN05 can reduce AQDS with sucrose, glucose, lactose, lactic acid, glycerol, acetic acid,ethanol, and methanol as electron donors, and the utilization capacity decreases in order. Strain HN05 can use sucrose as an electron donor to reduce the humic analog anthraquinone-1-sulfonate(α-AQS), sodium anthraquinone-2-sulfonate(AQS), AQDS, and sodium anthraquinone-1,5-disulfonic acid(1,5-AQDS). Strain HN05 can use sucrose as an electron donor to anaerobic degradation diquat, but the degradation rate is low;on the contrary, adding AQDS can greatly promote the degradation of diquat, and the degradation rate can reach 41.93% in 22 days. [Conclusions] This study reported for the first time that Kosakonia oryzae HN05 has the ability to reduce humus and degrade diquat, which enriched the strain resources and functional diversity of humus-reducing bacteria, and provided the basis for the restoration of diquat polluted environment.

关 键 词：腐殖质还原菌 Kosakonia oryzae 敌草快 厌氧降解 一级动力学拟合 

分 类 号：TQ450.2[化学工程—农药化工]