千年健的化学成分及抗肿瘤活性研究  

Study on the chemical constituents and anti-tumor activity of Homalomena occulta

作　　者：尹伟 胡琪[2] 李新章 周清浩 郁阳 胥振国 范高福[1] 张国升[5] YIN Wei;HU Qi;LI Xin-zhang;ZHOU Qing-hao;YU Yang;XU Zhen-guo;FAN Gao-fu;ZHANG Guo-sheng(School of Bioengineering,Hefei Vocational and Technical College,Hefei 238000,China;Chemical Composition Extraction,Separation and Identification Center of Nantong Ruifeng New Material Technology Co.,Ltd.,Nantong 226000,China;Department of Polymer Science and Engineering,University of Science and Technology of China,Chinese Academy of Sciences Key Laboratory of Material Chemistry,Hefei 230026,China;State Key Laboratory of Phytochemistry and Sustainable Utilization of Western Plant Resources,Kunming Institute of Botany,Chinese Academy of Sciences,Kunming 650201,China;School of Pharmacy,Anhui University of Traditional Chinese Medicine,Hefei 230031,China)

机构地区：[1]合肥职业技术学院生物工程学院,合肥238000 [2]南通睿沣新材料技术有限公司化学成分分离鉴定中心,南通226000 [3]中国科学技术大学高分子科学与工程系中科院软物质化学重点实验室,合肥230026 [4]中国科学院昆明植物研究所植物化学与西部植物资源持续利用国家重点实验室,昆明650201 [5]安徽中医药大学药学院,合肥230031

出　　处：《天然产物研究与开发》2022年第4期563-569,共7页Natural Product Research and Development

基　　金：国家自然科学基金(21875234);安徽省教育厅质量工程项目(2020zyk33);安徽省高校自然科学研究项目(KJ2021ZD0158);安徽省"双基"示范教研室“药学教研室”项目(2020jys005)。

摘　　要：为探讨千年健的化学成分及抗肿瘤活性,应用正相硅胶、中压制备液相、Sephadex LH-20等多种色谱分离手段,从千年健乙醇提液中分离得到10个化合物,依据波谱分析方法,所得化合物分别为桦木酸(1)、3β-acetoxyolean-12-en-28-oic acid(2)、熊果酸(3)、antriptolactone(4)、黄花菜木脂素C(5)、bullatantriol(6)、菠萝香藤素(7)、mucrolidin(8)、pinocembrin(9)、3-(4-hydroxy-3-methoxyphenyl)-propenal(10)。除化合物7、8外,本实验所分离得到的其余化合物,均为首次从千年健中分离。通过对化合物2、4、9与抗肿瘤药物甲氨蝶呤的体外细胞毒性及单细胞凝胶电泳对比试验表明,化合物2、4、9的抗肿瘤细胞效果显著,同时,上述化合物对荷瘤小鼠体内抗肿瘤对比试验结果,亦显示出各化合物具有较强抑制肿瘤组织生长作用,且化合物2、4、9对人肺癌细胞(A549)等4种肿瘤细胞株的研究为首次报道。In order to explore the chemical composition and anti-tumor activity of Homalomena occulta,10 compounds were separated from the ethanol extract of Homalomena occulta by using normal phase silica gel,medium-pressure liquid chromatography,Sephadex LH-20 and other chromatographic separation methods.According to spectral analysis method,the obtained compounds include betulinic acid(1),3β-acetoxyolean-12-en-28-oic acid(2),ursolic acid(3),antriptolactone(4),cleo-lignan C(5),bullatantriol(6),pineapple coriander(7),mucrolidin(8),pinocembrin(9),and 3-(4-hydroxy-3-methoxyphenyl)-propenal(10).Except for compounds 7 and 8,the remaining compounds isolated in this experiment were isolated from Homalomena occulta for the first time.The in vitro cytotoxicity and single-cell gel electrophoresis comparison tests of compounds 2,4,and 9 with the anti-tumor drug methotrexate showed that the anti-tumor cell effects of compounds 2,4,and 9 were significant.The anti-tumor comparative test in mice also showed that it has a strong inhibitory effect on tumor tissue growth.The study of compounds 2,4,and 9 against 4 kinds of tumor cell lines including human lung cancer cells(A549) is reported for the first time.

关 键 词：千年健 化学成分 抗肿瘤活性 结构鉴定 

分 类 号：R284.2[医药卫生—中药学]