酶辅助超声法提取陕产黄精总黄酮及其抗氧化、抗A549细胞增殖活性研究  

Enzymatic-ultrasonic assisted extraction of total flavonoids from Shaanxi Polygonatum sibiricum and in vitro evaluation of their anti-oxidant and anti-A549 proliferation activities

作　　者：郭凯丽 刘继平 赵重博[1] 史永恒 王斌 权利娜 过晓芳[4] 朱星枚[1] GUO Kai-li;LIU Ji-ping;ZHAO Chong-bo;SHI Yong-heng;WANG Bin;QUAN Li-na;GUO Xiao-fang;ZHU Xing-mei(Department of Pharmacology,Shaanxi University of Chinese Medicine;Key Laboratory of Pharmacodynamics and Material Basis of Chinese Medicine of Shaanxi Administration of Traditional Chinese Medicine;Shaanxi Key Laboratory of Traditional Medicine Foundation and New Drug Research,Xianyang 712046,China;School of Science,Xi an Technological University,Xi an 710021,China)

机构地区：[1]陕西中医药大学药学院 [2]陕西省中医药管理局中药药效机制与物质基础重点研究室 [3]陕西省中药基础与新药研究重点实验室,咸阳712046 [4]西安工业大学数理系,西安710032

出　　处：《天然产物研究与开发》2022年第4期630-638,共9页Natural Product Research and Development

基　　金：国家自然科学基金(62102304);陕西省教育厅重点实验室项目(21JS015);陕西中医药大学研究生创新项目(2020CX33);陕西中医药大学创新团队项目(2019-YL13)。

摘　　要：优化陕产黄精总黄酮提取工艺,体外考查黄精总黄酮的抗氧化、抗A549细胞增殖活性。以黄精总黄酮含量为指标,在单因素试验基础上,应用Box-Behnken响应面法优化陕产黄精总黄酮提取工艺,黄精总黄酮最优提取工艺:2%纤维素酶,90%乙醇,超声20 min,料液比1∶16(g/mL),该工艺下黄精总黄酮的得率为103.10±0.02μg/g,该法简便可行、重复性高。高效液相色谱(HPLC)法分析陕产黄精总黄酮,其中含槲皮素二水物6.43±0.25μg/g和黄芩素92.31±1.75μg/g。黄精总黄酮清除DPPH自由基、羟自由基和ABTS的EC_(50)分别是11.20、57.88和23.75μg/mL,说明黄精总黄酮具有较强的抗氧化能力。CCK-8法表明黄精总黄酮作用A549细胞24、48、72 h的IC_(50)分别是25.18、12.52和9.25μg/mL,黄精总黄酮可剂量和时间依赖性抗A549细胞增殖。The aim of this work was to investigate enzyme-assisted ultrasonic extraction of Shaanxi Polygonatum sibiricum and their anti-oxidant and anti-A549 proliferation activities in vitro.Based on the single factor test,Box-Behnken experimental was adopted including dependent variables(cellulase amount,ethanol concentration,ultrasonic time and solid-liquid ratio) and independent variable(yield of flavonoids).The results showed that the optimum conditions were cellulase amount 2%,ethanol concentration 90%,ultrasonic time 20 min,and solid-liquid ratio 1∶16(g/mL).The yield of total flavonoids reached 103.10 ± 0.02 μg/g.The method was simple,feasible and repeatable.High performance liquid chromatography(HPLC) analysis showed that the flavonoids contained 6.43±0.25 μg/g quercetin dihydrate and 92.31±1.75 μg/g baicalin.The EC_(50) of total flavonoids on scavenging DPPH free radical,hydroxyl free radical and ABTS were 11.20,57.88 and 23.75 μg/mL respectively,which indicated that total flavonoids from P.sibiricum had strong anti-oxidant capacity.CCK-8 tests showed that the IC_(50) of total flavonoids treating on A549 cells was 25.18,12.52 and 9.25 μg/mL at 24,48 and 72 h respectively,which indicated that total flavonoids from P.sibiricum could inhibit the proliferation of A549 cells in a dose-and time-dependent manner.

关 键 词：黄精 总黄酮 酶辅助超声 高效液相 抗氧化 抗增殖 

分 类 号：Q282.71[生物学—细胞生物学]