巴西酒神菊化学成分及其体外抑制MDA-MB-231细胞增殖作用  

Chemical constituents from Baccharis trimera and their inhibitory activities on the proliferation of MDA-MB-231 cells in vitro

作　　者：李影[1] 黄璐琦[2] 刘宏栋[1] 闵建新[3] 朱金华[3] 周小青[1] 李斌[1] LI Ying;HUANG Lu-qi;LIU Hong-dong;MIN Jian-xin;ZHU Jin-hua;ZHOU Xiao-qing;LI Bin(Academician Workstation,Jiangxi University of Chinese Medicine,Nanchang 330004,China;China Academy of Chinese Medical Sciences,Beijing 100700,China;College of Life Sciences,Jiangxi University of Chinese Medicine,Nanchang 330004,China)

机构地区：[1]江西中医药大学院士工作站,江西南昌330004 [2]中国中医科学院,北京100700 [3]江西中医药大学生命科学学院,江西南昌330004

出　　处：《中成药》2022年第4期1148-1156,共9页Chinese Traditional Patent Medicine

基　　金：中央本级重大增减支项目(2060302-1701-01);江西中医药大学院士工作站项目(ysgzz201802);江西中医药大学一流学科项目(JXSYLXK-ZHYA0143)。

摘　　要：目的研究巴西酒神菊的化学成分及其体外抑制MDA-MB-231乳腺癌细胞增殖作用。方法巴西酒神菊95%乙醇提取物采用硅胶、ODS、HPLC等进行分离纯化,根据理化性质及波谱数据鉴定所得化合物的结构。MTT法测定其体外抑制MDA-MB-231乳腺癌细胞增殖作用。结果从中分离得到25个化合物,分别鉴定为alnincanone(1)、β-alnincanyl(2)、foliasalaci A4(3)、desoxyarticulyn(4)、7α,15,18-trihydroxy-ent-cleroda-3-ene(5)、15,16-diacetoxy-7α,18-dihydroxy-ent-cleroda-3-ene(6)、15,16-epoxy-7α,18-dihydroxy-15-oxo-ent-cleroda-3-ene(7)、7α-hydroxy-3,13-clerodadiene-16,15,18,19-diolide(8)、6,6,7,8,9,10-hexahydro-7,8-dimethyl-7-[2-(tetrahydro-5-oxo-3-furanyl)ethyl]-1H-naphtho[1,8-c]furan-3(5H)-one(9)、7-[2-(2,5-dihydro-5-oxo-3-furanyl)ethyl]-6,6,7,8,9,10-hexahydro-9-hydroxy-7,8-dimethyl-1H-naphtho[1,8-c]furan-3(5H)-one(10)、7α-15-dihydroxy-ent-clerod-3-en-18,19-olide(11)、鼠尾草素(12)、5-羟基-7,3′,4′-三甲氧基黄酮(13)、5,7,4′-三羟基-3′,8-二甲氧基黄酮(14)、异泽兰素(15)、5-羟基-6,7,3′,4′-四甲氧基黄酮(16)、芦丁(17)、槲皮素(18)、山柰酚(19)、豆甾醇(20)、15-二十九烷醇(21)、β-谷甾醇(22)、对羟基苯甲酸乙酯(23)、丁香树脂醇(24)、表无羁萜醇(25)。化合物15~16在10μmol/mL时细胞存活率分别为(32.50±2.66)%、(24.77±2.32)%。结论化合物1、2、21为首次从酒神菊属植物中分离得到,化合物3~7、12~15、23~24为首次从巴西酒神菊中分离得到,化合物15~16具有体外抑制MDA-MB-231乳腺癌细胞增殖作用。AIM To study the chemical constituents from Baccharis trimera(Less.)DC and their inhibitory activity on the proliferation of breast cancer cell MDA-MB-231 in vitro.METHODS The 95%ethanol extract from B.trimera was isolated and purified by silica,ODS and preparative HPLC,then the structures of obtained compounds were identified by physicochemical properties and spectral data.Their inhibitory activity on the proliferation of breast cancer cell MDA-MB-231 in vitro was determined by MTT mothod.RESULTS Twenty-five compounds were isolated and identified as alnincanone(1),β-alnincanyl(2),foliasalacnsi A4(3),desoxyarticulyn(4),7α,15,18-trihydroxy-ent-cleroda-3-ene(5),15,16-diacetoxy-7α,18-dihydroxy-ent-cleroda-3-ene(6),15,16-epoxy-7α,18-dihydroxy-15-oxo-ent-cleroda-3-ene(7),7α-hydroxy-3,13-16,15∶18,19-diolide)(8),6,6,7,8,9,10-hexahydro-7,8-dimethyl-7-[2-(tetrahydro-5-oxo-3-furanyl)ethyl]-1H-naphtho[1,8α-c]furan-3(5H)-one(9),7-[2-(2,5-dihydro-5-oxo-3-furanyl)ethyl]-6,6,7,8,9,10-hexahydro-9-hydroxy-7,8-dimethyl-1H-naphtho[1,8α-c]furan-3(5 H)-one(10),7α-15-dihydroxy-ent-clerod-3-en-18,19-olide(11),salvigenin(12),5-hydroxy-7,3′,4′-trimethoxyflavone(13),5,7,4′-trihydroxy-3,8-dimethoxy flavone(14),eupatilin(15),5-hydroxy-6,7,3′,4′-tetramethoxyflavone(16),rutin(17),quercetin(18),kaempferol(19),stigmasterol(20),15-nonacosanol(21),β-sitosterol(22),4-hydroxycinnamic acid methyl ester(23),syringaresinol(24),epifriedelanol(25).The cell survival rates of compounds 15-16 at 10μmol/mL were(32.50±2.66)%and(24.77±2.32)%,respectively.CONCLUSION Compounds 1-2,21 are isolated from genus Baccharis for the first time,and compounds 3-7,12-15,23-24 are first isolated from this plant.Compounds 15-16 exhibit inhibitory activities on the proliferation of breast cancer cell MDA-MB-231 in vitro.

关 键 词：巴西酒神菊 化学成分 分离鉴定 MDA-MB-231细胞 

分 类 号：R284􀆰[医药卫生—中药学]