葡萄Golden2-like转录因子家族的鉴定及其表达分析  

Identification and expression analysis of the Golden2-like transcription factor family of Vitis vinifera L.

作　　者：许晨 李青云[2,3] 万俊男 侯雨君 周慧敏 朱振飞 辛海平 李吉涛[1] Xu Chen;Li Qinq-Yun;Wan Jun-Nan;Hou Yu-Jun;Zhou Hui-Min;Zhu Zhen-Fei;Xin Hai-Ping;Li Ji-Tao(School of Forestry and Horticulture,Hubei Minzu University,Enshi,Hubei,445000;Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture,Wuhan Botanical Garden,Chinese Academy of Sciences,Wuhan 430074,China;Sino-Africa Joint research Center,Chinese Academy of Sciences,Wuhan 430074,China)

机构地区：[1]湖北民族大学林学园艺学院,湖北恩施445000 [2]中国科学院武汉植物园,中国科学院植物种植创新与特色农业重点实验室,武汉430074 [3]中国科学院中-非联合研究中心,武汉430074

出　　处：《植物科学学报》2022年第2期205-215,共11页Plant Science Journal

基　　金：湖北省自然科学基金(2021CFB555);湖北民族大学博士启动基金(MD2019B016)。

摘　　要：本研究对葡萄(Vitis vinifera L.)的Golden2-like(GLK)转录因子家族进行了全基因组鉴定和表达模式分析,并利用品种‘玫瑰香’(V.vinifera cv.Muscat Hamburg)进一步验证其在低温胁迫下的响应。结果显示,葡萄Golden2-like家族共46个成员,分为5个亚族,同一亚族的保守结构域相似。46个VvGLK分别定位于细胞核、叶绿体、细胞质和过氧化物酶体中,其启动子区域含多种逆境应答顺式作用元件。基因芯片分析结果表明,22个Golden2-like基因在果实发育过程中变化显著。同时,有15、15和9个基因分别响应盐、干旱和低温胁迫。qRT-PCR分析发现26个基因参与低温应答。VvGLK41在所有胁迫处理中均下调表达。In this study,the Golden2-like transcription factor(TF)gene family was identified and analyzed in Vitis vinifera L.We further verified the expression patterns of Golden2-like genes under low temperature in V.vinifera‘Muscat Hamburg’seedlings.Results showed that 46 Golden2-like genes were identified in V.vinifera and classified into five subgroups.The conserved domain was similar within the same subgroup.Forty-six VvGLKs were located in the nucleus,chloroplast,cytoplasm,and peroxisome,and their promoter regions contained a variety of cis-elements related to stress response.Gene chip data showed that 22 VvGLKs changed significantly during fruit development and 15,15,and 9 VvGLKs were expressed after salt,drought,and low-temperature stress,respectively.qRT-PCR identified 26 VvGLKs involved in cold-stress response.Notably,VvGLK41 was down-regulated after salt,drought,and low-temperature treatment.Thus,this study provides clues for fruit development and stress response in grape.

关 键 词：葡萄 Golden2-like 低温胁迫 果实发育 表达分析 

分 类 号：Q943.2[生物学—植物学]