射干苷对肺腺癌细胞迁移的影响及机制研究  

Effect of Tectoridin on migration of lung adenocarcinoma cells and the mechanism

作　　者：黄禹舒 陈仁杰 陆锐 谢燃 吴志浩[1,2] HUANG Yushu;CHEN Renjie;LU Rui;XIE Ran;WU Zhihao(Research Laboratory of Tumor Microenvironment,Wannan Medical College,Wuhu 241002,China)

机构地区：[1]皖南医学院肿瘤微环境研究室,安徽芜湖241002 [2]皖南医学院医学生物学教研室,安徽芜湖241002

出　　处：《皖南医学院学报》2022年第2期108-112,共5页Journal of Wannan Medical College

基　　金：国家自然科学基金项目(81872371);安徽省自然科学基金项目(1708085MH203);分子肿瘤学国家重点实验室开放课题(SKL-KF-2019-11)。

摘　　要：目的:研究射干苷对肺腺癌细胞系H299及A549细胞迁移的影响及作用机制。方法:噻唑蓝(MTT)细胞活力检测射干苷对细胞增殖的影响;流式细胞术检测细胞凋亡情况;细胞划痕实验探究射干苷对细胞迁移能力的影响;ROS活性氧试剂盒测定射干苷对细胞内源活性氧生成的影响;Western blot检测射干苷处理对细胞PI3K/AKT蛋白及EMT相关蛋白的表达水平。结果:MTT实验结果表明,随着射干苷处理浓度提高和时间推移细胞增殖率均下降(P<0.05);流式细胞术测凋亡显示射干苷可促进细胞凋亡(P<0.05);细胞划痕实验结果表明,射干苷对细胞迁移能力明显抑制(P<0.05);ROS活性氧测定显示射干苷导致细胞ROS活性氧上升;Western blot结果显示,随药物浓度增高,p-AKT蛋白表达降低,上皮标记蛋白E-cadherin表达增多,而间质标记蛋白Fibronectin及Snail蛋白表达减少。结论:在肺腺癌细胞中,射干苷激发ROS抑制PI3K/AKT通路,从而下调Snail表达,抑制EMT的发生,从而减少肿瘤细胞迁移。Objective:To investigate the effect of Tectoridin on migration of lung adenocarcinoma cell lines h299 and A549 and the mechanisms.Methods:MTT assay was used to detect the effect of Tectoridin on the cell proliferation,and flow cytometry was used to measure the cell apoptosis.Cell scratch test was performed to observe the effect of Tectoridin on cell migration.ROS kit was used to determine the effect of Tectoridin on the production of endogenous reactive oxygen species,and the expression levels of PI3K/AKT and EMT related proteins were measured with Western blotting.Results:MTT showed that the cell proliferation rate was decreased with added Tectoridin concentration and time(P<0.05).Flow cytometry demonstrated that Tectoridin could promote the cell apoptosis(P<0.05),and cell scratch test indicated that Tectoridin significantly inhibited the cell migration(P<0.05).The results of ROS showed that Tectoridin increased ROS in the lung adenocarcinoma cells,and Western blot results showed that with the increase of drug concentration,the expression of p-AKT was decreased,yet the expression of E-cadherin was up-regulated,and the expression of fibronectin and Snail was down-regulated.Conclusion:Tectoridin can induce ROS to inhibit PI3K/AKT pathway in the lung adenocarcinoma cells,thereby down regulating Snail expression and inhibiting EMT,eventually leading to reduced tumor cell migration.

关 键 词：射干苷 肺腺癌 迁移 PI3K/AKT EMT 

分 类 号：R285[医药卫生—中药学]