郁金配伍丁香对小鼠肝纤维化影响的实验研究  

Experimental study on the effect of Curcumae Radix combined with Flos Caryophylli on hepatic fibrosis in mice

作　　者：汪居安 宋雪敏 王俐晴 王亚奇 谢露红 汪炜 王健君[2] WANG Ju′an;SONG Xuemin;WANG Liqing;WANG Yaqi;XIE Luhong;WANG Wei;WANG Jianjun(School of Clinical Medicine,Wannan Medical College,Wuhu 241002,China)

机构地区：[1]皖南医学院临床医学院,安徽芜湖241002 [2]皖南医学院组织学与胚胎学教研室,安徽芜湖241002

出　　处：《皖南医学院学报》2022年第2期187-189,204,共4页Journal of Wannan Medical College

基　　金：安徽省大学生创新创业训练计划项目(S201910368025)。

摘　　要：目的:探究郁金配伍丁香对肝纤维化小鼠的影响。方法:SPF级小鼠32只,随机等分为郁金组、丁郁组、空白组、模型组;10%CCl_(4)的橄榄油溶液腹腔注射建立肝纤维化模型,同时给予中药灌胃处理。持续7周后取血清检测ALT、AST、HA、IL-6、SOD、MDA含量,并取肝右叶行病理检测。结果:模型组的血清ALT、AST、HA、IL-6、MDA水平均高于空白组(P<0.05),肝细胞结构模糊,细胞变性坏死并伴有炎细胞浸润与胶原纤维沉积。郁金组、丁郁组的血清ALT、AST、HA、IL-6、MDA水平均低于模型组而高于空白组(P<0.05),丁郁组的血清AST、HA、IL-6水平低于郁金组(P<0.05),郁金组、丁郁组的血清SOD水平均高于空白组和模型组(P<0.05)。郁金组和丁郁组与模型组相比,细胞变性坏死、炎细胞浸润与胶原纤维沉积的数量和范围均有较大改善;郁金组和丁郁组两者比较,肝细胞变性坏死程度与范围相近,但后者炎细胞浸润与胶原沉积程度较前者轻。结论:CCl_(4)生成自由基与炎症反应造成肝纤维化,郁金单用或合用丁香均能通过消除自由基和减轻炎症反应干预纤维化,丁香能够增强郁金抗炎和抗纤维化的能力。Objective:To observe the effect of Curcumae Radix combined with Flos Caryophylli on hepatic fibrosis in mice.Methods:Thirty-two SPF mice were randomly divided into Curcumae Radix group,Flos Caryophylli group,blank control group and model group.Hepatic fibrosis models were established by intraperitoneal injection of 10%CCl_(4) in olive oil solution,and underwent simultaneous intragastric administration with the Chinese medicine.After 7 weeks,serum ALT,AST,HA,IL-6,SOD and MDA were measured,and right lobe of liver was taken for pathological examination.Results:Serum levels of ALT,AST,HA,IL-6 and MDA were significantly higher in model group than in blank control group(P<0.05).The structure of hepatocytes was fuzzy,and the hepatocytes were denatured and necrotic,with inflammatory infiltration and deposition of collagen fibers.Serum ALT,AST,HA,IL-6 and MDA levels in simple Curcumae Radix group and Flos Caryophylli-Curcumae Radix group were significantly lower than those in model group,yet higher than those in blank control group(P<0.05).Serum AST,HA and IL-6 levels were lower in Flos Caryophylli-Curcumae Radix group than in simple Curcumae Radix group(P<0.05),yet the serum SOD level was markedly higher in Curcumae Radix group and Flos Caryophylli-Curcumae Radix group than in blank control group and model group(P<0.05).The number and range of hepatocytic degeneration and necrosis,inflammatory infiltration as well as deposition of collagen fibers were significantly improved in the Curcumae Radix group and Flos Caryophylli-Curcumae Radix group compared with the model group.However,the degree and range of hepatocytic degeneration and necrosis were similar between the Flos Caryophylli-Curcumae Radix group and simple Curcumae Radix group,whereas the severity of inflammatory infiltration and deposition of collagen fibers in the Flos Caryophylli-Curcumae Radix group was less than that in the Curcumae Radix group.Conclusion:The formation of free radicals of CCl_(4) and inflammation may cause hepatic fibrosis.Single Curcumae Rad

关 键 词：丁香 郁金 “十九畏” 肝纤维化 

分 类 号：R285.5[医药卫生—中药学]