磷高效转基因水稻OsPT4根际高效解有机磷细菌的分离鉴定  

Isolation and identification of efficient organophosphate-dissolving bacteria from rhizosphere soil of P-efficient transgenic rice OsPT4

作　　者：张乐 张思宇 王亚露 李刚 杨殿林 赵建宁 ZHANG Le;ZHANG Siyu;WANG Yalu;LI Gang;YANG Dianlin;ZHAO Jianning(Agro-Environmental Protection Institute,Ministry of Agriculture and Rural Affairs,Tianjin 300191,China;Key Laboratory of Environmental Pollution Prevention and Control in Production Area,Ministry of Agriculture and Rural Affairs/Tianjin Key Laboratory of Agricultural Environment and Agricultural Product Safety,Tianjin 300191,China)

机构地区：[1]农业农村部环境保护科研监测所,天津300191 [2]农业农村部产地环境污染防控重点实验室/天津市农业环境与农产品安全重点实验室,天津300191

出　　处：《农业环境科学学报》2022年第4期834-840,共7页Journal of Agro-Environment Science

基　　金：国家转基因重大专项(2016ZX08012005-005);中国农业科学院科技创新工程协同创新任务(CAAS-XTCX2016015)。

摘　　要：为提升磷高效转基因水稻根际土壤的磷素利用率,采用传统微生物分离培养技术分离筛选高效解有机磷菌株,并对分离菌株进行鉴定和解磷能力的测定。以改良的蒙金娜固体有机磷培养基分离磷高效转基因水稻OsPT4根际土壤中的解有机磷菌株,检测其溶磷特性,并通过比对16S rDNA基因序列进行分类鉴定。结果表明:从磷高效转基因水稻根际土壤中分离获得15株解菌株,纯化复筛后获得6株可以产生明显溶磷圈且生长稳定的解有机磷菌株。6株解磷菌的可溶性指数在1.50~6.33之间,其中菌株Y7的可溶性指数最高;培养24 h后菌株的解磷量在10.60~87.43 mg·L^(-1)之间,其中菌株Y7最大。培养24 h后菌株Y7的菌液pH降低最多,碱性磷酸酶分泌量仅低于菌株Y11。除Y7外的5株菌株均为大田土壤中分离筛选的常见类群,在解磷能力、溶磷特性上与传统大田作物中筛选获得的解磷菌株无明显差异。菌株Y7的解磷能力最强,经鉴定其属于泛菌属(Pantoea sp.),是常见的水稻种子内生菌核心类群,酸化作用和碱性磷酸酶作用是其主要的解磷机制,Y7可作为高效解磷菌资源进行进一步研究。P-efficient transgenic rice OsPT4 shows a relatively high consumption rate of available phosphorus.To increase P-utilization,organophosphate-dissolving bacteria were isolated from the rhizosphere soil of P-efficient transgenic rice OsPT4 by Pikovskaya organophosphorus solid medium and classified by 16S rDNA gene sequencing.Fifteen organophosphate-dissolving strains were isolated,of which six produced clear transparent circles on the organophosphorus medium and were used for purification and rescreening.Their solubility index ranged from 1.50~6.33,and strain Y7 showed the highest solubility.Phosphate-dissolving capacity,after being cultured for 24 h,ranged from 10.60~87.43 mg·L^(-1),and strain Y7 showed the highest capacity.After culturing for 24 h,strain Y7 showed the highest pH reduction,and the second highest alkaline phosphatase secretion(after strain Y11).Five of the strains(excluding Y7)were common groups that could be screened from traditional field crop soil and they showed no difference in P-solubilizing ability or mechanism.Strain Y7 was identified as Pantoea sp.,a common core group of endophytic bacteria in rice seeds.Its phosphorolytic mechanism was the interaction of pH reduction and alkaline phosphatase secretion,which could be used as a resource for efficient phosphorolytic bacteria in future research.

关 键 词：磷高效转基因水稻 解有机磷细菌 16Sr DNA 

分 类 号：S511[农业科学—作物学]