菌核病不同发病率的菜田土壤中真菌多样性分析  

Analysis of fungal diversity in vegetable field with different incidence rate of Sclerotinia sclerotiorum

作　　者：陶禹 李东波 李兴杰 陈士亮 严振宁 奥岩松[1] TAO Yu;LI Dongbo;LI Xingjie;CHEN Shiliang;YAN Zhenning;AO Yansong(School of Agriculture and Biology,Shanghai Jiao Tong University,Shanghai 200240,China;Colloge of Life Science Resources and Environment,Yichun University,Yichun 336600,China;Shanghai Chunchang Vegetable and Fruit Cooperative,Shanghai 201706,China)

机构地区：[1]上海交通大学农业与生物学院,上海200240 [2]宜春学院生命科学与资源环境学院,宜春336600 [3]上海春昌蔬果专业合作社,上海201706

出　　处：《上海农业学报》2022年第2期63-69,共7页Acta Agriculturae Shanghai

基　　金：上海市科技兴农重点攻关项目[沪农科创字(2020)第2-1号]。

摘　　要：以菌核病不同发病率的菜田土壤为材料,采用Illumina MiSeq测序技术分析土壤真菌群落结构组成和多样性变化,探讨土壤真菌与小核盘菌的互作关系。结果表明:菌核病发病率不同的菜田土壤中,小核盘菌使土壤真菌丰富度Sobs指数显著下降、土壤中真菌群落组成以及Beta多样性产生显著变化,其中重度组中特有蛙粪霉门(Basidiobolomycota)、芽枝霉门(Blastocladiomycota)和Calcarisporiellomycota,轻度病害组中特有蛙粪霉门(Basidiobolomycota)和芽枝霉门(Blastocladiomycota)。土壤中的小被孢霉属(Mortierella)和丝孢酵母属(Trichosporon)对病原菌均具有拮抗作用,具有分离出高抑制性的菌株的潜力,同时丝孢酵母属也可作为核盘菌侵染时的指示真菌。In order to elucidate the interaction between Sclerotinia minor and soil fungi,soil fungi community structure and richness were sequenced by second generation high-throughput sequencing method,using vegetable field soils with different incidence rate of Sclerotinia sclerotiorum as materials.The results were as follows:(1)Sclerotinia significantly decreased the sobs index of soil fungal richness,and significantly changed the composition of fungal community and beta diversity in soil.(2)Basidiobolomycota,Blastocladiomycota and Calcarisporiellomycota were endemic in severe disease group.Basidiobolomycota and blastocladiomycota were endemic in light desease group.(3)Mortierella and Trichosporon in soil might have antagonistic effects on pathogens and have the potential to isolate highly inhibitory strains.Meanwhile,Trichosporon could be also used as an indicator of Sclerotinia infection.

关 键 词：菌核病 真菌多样性分析 小核盘菌 菜田土壤 群落结构 

分 类 号：S435[农业科学—农业昆虫与害虫防治]