柴胡疏肝散加味江香薷对SD大鼠28天喂养实验研究  

An Experimental Study on 28 d Feeding of Chaihu Shugan Powder and Mosla Chinensis Maxim SD Rats

作　　者：陈雨然 罗琪 罗黎明 李龙雪 舒坤 徐磊 洪滔 杨玉艳 曾静军 彭赐玲 刘志勇 CHEN Yuran;LUO Qi;LUO Liming;LI Longxue;SHU Kun;XU Lei;HONG Tao;YANG Yuyan;ZENG Jingjun;PENG Cling;LIU Zhiyong(Laboratoa Animol Science ond Technology Center,Jiangxi University of Traditional Chinese Medicine,330004,Nanchang,PRC)

机构地区：[1]江西中医药大学实验动物科技中心,南昌330004

出　　处：《江西科学》2022年第2期253-257,共5页Jiangxi Science

基　　金：江西中医药大学双一流学科项目(JXSYLXK-ZHYI025);江西中药大学大学生2021年创新创业项目。

摘　　要：观察柴胡疏肝散加味江香薷各剂量对幼龄SD大鼠生理、生化等指标的影响,并应用损益指数-总积分(BDI-GS)体系进行安全性评估,为柴胡疏肝散加味江香薷的临床用药提供依据。准备SPF级SD幼龄大鼠80只,按体重分层随机分为4组,即柴胡疏肝散加味江香薷低(0.2 g/kg)、中(2 g/kg)、高(20 g/kg)剂量组和空白对照组(水,0 mg/kg),每组20只,雌雄各半。每天灌胃给药1次,连续给药28 d,实验期间,每天对大鼠进行一般状态观察,每周记录一次体重,于第29天对大鼠进行麻醉采血及各脏器解剖取材。对大鼠体重、血液常规、血清生化、尿常规、主要脏器的组织病理学检查等指标进行检测。结果:实验期间给药组大鼠的行为、活动、毛发颜色、对外反应、粪便性质与空白对照组比较无明显差异;体重结果表明柴胡疏肝散加味江香薷各剂量组大鼠体重增长与空白对照组无显著性差异;血清生化结果显示雌性中剂量组大鼠AST水平较空白对照组显著降低,结合肝脏病理学观察,该指标下降无毒理学意义;血常规指标中给药组与空白对照组无显著性差异;尿常规结果和肾脏病理学观察中未见变化,且血清生化中与肾功能相关的指标均未见异常;雌性大鼠的各脏器总累计GS值随着给药剂量的增加呈上升的趋势,在高剂量时GS值>10,雄性大鼠的各脏器总累计GS值在中剂量时>10。综合各项检测结果得出柴胡疏肝散加味江香薷对SD大鼠无毒性作用。To observe the effects of each dose of Chaihu Shugan Powder plus Mosla chinensis Maxim on the physiological and biochemical indicators of juvenile SD rats,conduct safety evaluation,and provide a basis for the clinical use of Chaihu Shugan Powder plus Mosla chinensis Maxim.Prepare80 SPF-level SD young rats and randomly divide them into 4 groups according to their body weight,namely,Chaihu Shugan Powder plus Mosla chinensis Maxim low(0.2 g/kg),medium(2 g/kg),and high(20 g/kg).The dose group and the blank control group(water,0 mg/kg),each group has 20 animals,half of the male and the female.Gavage was administered once a day for 28 consecutive days.During the experiment,the rats were observed in their general state every day,and their body weight was recorded once a week.On the 29th day,the rats were anesthetized for blood collection and various organs were dissected.The rats’weight,blood routine,serum biochemistry,urine routine,benifit damage index-general score(BDI-GS),histopathological examination of main organs and other indicators were evaluated.Results:During the experiment,the behavior,activity,hair color,external reaction,and fecal properties of the rats in the administration group were not significantly different from those in the blank control group;The body weight results showed that the weight gain of rats in each dose group of Chaihu Shugan Powder plus Mosla chinensis Maxim was not significantly different from that of the blank control group;Serum biochemical results showed that the AST level of rats in the female medium-dose group was significantly lower than that in the blank control group,but there was no change in liver pathology,indicating that Chaihu Shugan Powder had an effect on the liver of rats,but it had no toxic effect;There is no significant difference between the administration group and the blank control group in hematological indicators;The results of urine routine and renal pathology showed no change in each group.The total cumulative GS value of each organ of female rats increased wi

关 键 词：柴胡疏肝散 江香薷 安全性评价 

分 类 号：R285.5[医药卫生—中药学]