高、低相对分子质量再生丝素蛋白改性丝素纤维人工血管材料的探究  

Exploration on silk fibroin fiber artificial vascular materials modified by regenerated silk fibroin protein with high or low relative molecular mass

作　　者：于成龙[1,2,3] 秦金桥 关国平 Turng Lih-sheng[3] 王璐[1,2] Yu Chenglong;Qin Jinqiao;Guan Guoping;Turng Lih-sheng;Wang Lu(College of Textiles,Donghua University,Shanghai 201620,China;Key Laboratory of Textile Science and Technology of Ministry of Education,Donghua University,Shanghai 201620,China;Wisconsin Institute for Discovery,University of Wisconsin-Madison,Madison WI 53705,USA)

机构地区：[1]东华大学纺织学院,上海201620 [2]东华大学纺织面料技术教育部重点实验室,上海201620 [3]威斯康星大学麦迪逊分校威斯康星探索研究所,美国麦迪逊53705

出　　处：《产业用纺织品》2022年第1期22-32,共11页Technical Textiles

基　　金：中央高校基本科研业务费专项(2232019G-06);上海市教育委员会科研创新项目(ZX201503000017);高等学校学科创新引智计划资助(BP0719035)。

摘　　要：采用高、低相对分子质量再生丝素蛋白对He低温等离子体处理的丝素纤维人工血管材料进行表面改性,研究其对丝素纤维人工血管材料生物相容性问题的改善状况。结果显示,接枝再生丝素蛋白可以有效地提升丝素纤维人工血管材料的拉伸断裂强度、初始模量及疏水性。综合考量接枝效果,确定优选质量分数为1%的高相对分子质量再生丝素蛋白溶液和质量分数为5%的低相对分子质量再生丝素蛋白溶液对材料接枝改性。蛋白吸附、溶血率和细胞毒性测试结果显示,相较于低相对分子质量再生丝素蛋白,高相对分子质量再生丝素蛋白改性丝素纤维人工血管材料的生物相容性改善效果更为显著。Regenerated silk fibroin protein with high or low relative molecular mass was used to modify the surface of silk fibroin fiber artificial vascular materials treated by He low temperature plasma,and the improvement of biocompatibility of silk fibroin fiber artificial vascular materials was studied.The results showed that grafting regenerated silk fibroin protein could effectively improve the tensile breaking strength,initial modulus and hydrophobicity of silk fibroin fiber artificial vascular materials.After comprehensive consideration of grafting effect,1wt%high relative molecular mass regenerated silk fibroin protein solution and 5wt%low relative molecular mass regenerated silk fibroin protein solution were optimized for grafting modification.The results of protein adsorption,hemolysis rate and cytotoxicity test showed that,the biocompatibility of silk fibroin fiber artificial vascular materials modified with high relative molecular mass regenerated silk fibroin protein was more significant than that modified with low relative molecular mass regenerated silk fibroin protein.

关 键 词：再生丝素蛋白 丝素纤维 相对分子质量 接枝改性 蛋白吸附 溶血率 细胞毒性 生物相容性 

分 类 号：R318.11[医药卫生—生物医学工程]