中华绒螯蟹(Eriocheir sinensis)幼蟹上岸病的免疫学研究  

IMMUNOLOGICAL STUDIES ON THE DISEMBARKATION SYNDROME OF JUVENILE CHINESE MITTEN-HANDED CRAB ERIOCHEIR SINENSIS

作　　者：宋林生[1] 苏建国[2] 崔朝霞[1] 方敏[1] 蔡中华[1] 丁茂昌 杨公社[3] 

机构地区：[1]中国科学院海洋研究所,青岛266071 [2]山东中华绒螯蟹繁育有限公司,东营257000 [3]西北农林科技大学,陕西杨陵712100

出　　处：《海洋与湖沼》2002年第6期657-662,共6页Oceanologia Et Limnologia Sinica

基　　金：国家重点基础研究发展规划项目G1 9990 1 2 0 0 7号

摘　　要：对患上岸病幼蟹和正常幼蟹体内的超氧化物歧化酶 (SOD)、过氧化氢酶 (CAT)、溶菌酶活性及丙二醛含量等几项免疫学指标的测定和比较 ,探讨幼蟹上岸死亡疾病的病因。结果表明 ,患病幼蟹自由基的代谢出现紊乱 ,清除自由基的能力较正常幼蟹显著下降 ,其中超氧化物歧化酶及过氧化氢酶的活性极显著低于正常幼蟹 (P <0 .0 1 )。而在反映自由基损伤程度的丙二醛含量上 ,患病幼蟹极显著高于正常幼蟹 (P <0 .0 1 ) ;患病幼蟹的溶菌酶活性显著低于对照组 (P <0 .0 5 )。研究表明 ,大量自由基的积累对幼蟹的组织或器官造成了损伤 ,同时也降低了机体的免疫能力 。Immunological approaches were used to measure the enzyme activity such as Superoxide dismutase (SOD), Catalase (CAT) and Lysozyme (LSZ), and the content of Malondiadehyde (MDA) in the disembarkation syndrome juvenile and the normal juvenile of Chinese mitten handed crab, Eriocheir sinensis. Maladjustment of free radicals metabolism was obvious in the disembarkation syndrome juvenile, and the ability of eliminating free radicals in the disembarkation syndrome juvenile decreased greatly. SOD and CAT, reflecting the ability of eliminating free radicals in the body, were both greatly significantly lower than those of the control group ( P <0.01); the content of MDA, reflecting free radicals damnification degree in the body, was greatly significantly higher than that in the control group ( P <0.01). The activity of LSZ in the disembarkation syndrome juvenile was significantly lower than that of the control group ( P <0.05). The results indicated that the free radicals played an important role in the break out of the juvenile disembarkation syndrome and resulted in immunity declining and physiological damage.

关 键 词：中华绒螯蟹 幼蟹上岸病 超氧化物歧化酶 过氧化氢酶 丙二醛 溶菌酶 养殖 

分 类 号：S947.9[农业科学—水产养殖] S942.[农业科学—水产科学]