泡沫传输法制备嘧菌酯/聚乳酸微球  

Preparation of Azoxystrobin/Polylactic Acid Microspheres via Foam-Transfer

作　　者：韩万青 何艳萍[1] 李贵营 潘孝霖 王芸 HAN Wanqing;HE Yanping;LI Guiying;PAN Xiaolin;WANG Yun(Faculty of Chemical Engineering,Kunming University of Science and Technology,Kunming 650500,China)

机构地区：[1]昆明理工大学化学工程学院,云南昆明650500

出　　处：《昆明理工大学学报（自然科学版）》2022年第2期27-34,共8页Journal of Kunming University of Science and Technology(Natural Science)

基　　金：国家自然科学基金项目(22068018,21466016);云南省“万人计划”-青年拔尖人才项目。

摘　　要：采用一种新型的泡沫夹带传输技术制备聚乳酸(Polylactic Acid,PLA)微球,考察了该制备方法中油水比、转速、聚乙烯醇(Polyvinyl Alcohol,PVA)浓度等条件对微球收率、形貌、粒径等的影响规律;在此基础上使用该方法制备了嘧菌酯/聚乳酸微球,考察了致孔剂用量对微球载药性能的影响并研究了嘧菌酯/聚乳酸微球的缓释动力学.实验结果表明:采用泡沫夹带传输技术,可以成功制备聚乳酸微球和嘧菌酯/聚乳酸微球并能实现连续化生产.在制备聚乳酸微球时,当油水质量比≥30∶100、转速≥600 r/min、水相中PVA浓度≥1.0%时,微球收率≥89.09%.制备嘧菌酯/聚乳酸微球时,不引入致孔剂正庚烷(n-heptane,HT)时,微球的包封率≥86.54%,载药量≥17.31%.伴随着致孔剂正庚烷引入含量的增加,包封率和载药量逐渐降低.不同致孔剂含量的嘧菌酯/聚乳酸微球缓释动力学均符合Korsmeyer-Peppas模型,n<0.45,为菲克扩散.A novel foam-transfer method was used to prepare Polylactic Acid(PLA) microspheres.The effects of oil-water ratio,stirring rate,Polyvinyl Alcohol(PVA) concentration in aqueous phase on yield,morphology and structure and particle size of PLA microspheres were studied.Moreover,azoxystrobin/PLA microspheres were prepared by using this method,and the effects of porogen content on the encapsulation efficiency and drug loading of azoxystrobin/PLA microspheres were investigated.At last,the sustained release kinetics of azoxystrobin/PLA microspheres were investigated.The results showed that both PLA microspheres and azoxystrobin/PLA microspheres can be prepared continuously successfully by using the novel method.When the oil-water ratio ≥ 30 ∶100,the stirring velocity ≥ 600 r/min,and the PVA concentration ≥1.0%,the yield of PLA microspheres in the receiving vessel ≥ 89.09%.When no n-heptane was introduced,the encapsulation efficiency and drug loading of azoxystrobin/PLA microspheres were 86.54% and 17.31%.The encapsulation efficiency and drug loading of azoxystrobin/PLA microspheres decreased as the content of n-heptane increased.The sustained release kinetics of azoxystrobin/PLA microspheres with different porogen content all conformed to the Korsmeyer-Peppas model,in which n<0.45,indicating that it was Fick diffusion.

关 键 词：聚乳酸微球 连续化制备 泡沫 嘧菌酯 药物释放 

分 类 号：TQ460.4[化学工程—制药化工] R943[医药卫生—药剂学]