吸附-生物耦合技术除磷的影响机制及微生物群落分析  

The Influence Mechanism of Adsorption-Biocoupling Technology on Phosphorus Removal and Microbial Community Analysis

作　　者：周西兵 陈孟林[1,2] 宿程远[1,2] 黄智 唐华君[1,2] 王连荧 ZHOU Xibing;CHEN Menglin;SU Chengyuan;HUANG Zhi;TANG Huajun;WANG Lianying(School of Environment and Resources,Guangxi Normal University;Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection,Ministry of Education,Guilin 541004,China)

机构地区：[1]广西师范大学环境与资源学院 [2]珍稀濒危动植物生态与环境保护教育部重点实验室,广西桂林541004

出　　处：《水处理技术》2022年第5期70-75,80,共7页Technology of Water Treatment

基　　金：国家自然科学基金项目(51768009);珍稀濒危动植物生态与环境保护教育部重点实验室基金项目。

摘　　要：研究了以焦炭为填料的吸附柱在不同吸附量和氧环境下对磷去除量的变化情况,并结合对微生物群落的分析,以揭示进水浓度、供氧方式和供氧量对吸附-生物耦合技术气化除磷的影响机制。结果表明,在进水P质量浓度为25 mg/L时,吸附柱对P的去除效果最好,单日生物降解量、脂磷生物量、脱氢酶活性和比生物活性分别为5.85 mg/d、73.76 nmol/g、4.64μgTF/(g·h)和0.063μgTF/(nmolP·h),进水P浓度过大会导致焦炭上的生物膜过厚,生物活性降低。前半程供气,后半程供气和全程供气时对P的去除率分别为60.44%、65.78%和63.65%。供气速率分别为10、100、300 mL/min和不供气时对P的去除率分别为40.98%、43.98%、39.80%和31.92%。少量供气能提高系统对磷的去除效率,不供气或供气量过大会对部分微生物产生抑制作用。对比各个吸附柱中微生物群落组成发现,有多种微生物被证实具有代谢有机物和脱氮除磷的作用。门水平上相对丰度较大的优势菌门有Proteobacteria(7.04%~70.33%)、Firmicutes(0.91%~47.13%)、Actinobacteria(9.06%~55.47%)和Bacteroidetes(6.49%~22.03%),其中Proteobacteria的丰度变化趋势与P的去除率呈正相关。属水平上相对丰度较大菌属有Burkholderia(0.01%~31.5%)、Clostridium_sensu_stricto(0.45%~34.47%)、Pandoraea(0.63%~17.11%)、Sphingomonas(0.48%~18.83%)、Corynebacterium(6.36%~53.41%)、Rhodobacter(0.01%~18.82%)和unclassified_Propionibacteriaceae(0.48%~34.91%)。The changes of phosphorus removal from coke-filled adsorbents under different adsorption and oxygen conditions were studied.And the influence mechanism of influent concentration,oxygen supply mode,and oxygen supply on phosphorus removal by the adsorption-biological coupling technology was revealed based on the analysis of the microbial community.The results showed that the adsorption column had the best removal effect and the daily biodegradation,lipid-phosphorus biomass,dehydrogenase activity,and specific bioactivity of the column were 5.85 mg/d,73.76 nmol/g,4.64μgTF/(g·h),and 0.063μgTF/(nmolP·h)under the influent P concentration was 25 mg/L.The influent P concentration was too high,leading to more thickness of biofilm on the coke,and the bioactivity was decreased.The removal rate of P in the first half,the second half,and the whole air supply were 60.44%,65.78%,and 63.65%respectively.When the gas supply rates were 10,100,300 mL/min or no gas supply,the P removal rate was 40.98%,43.98%,39.80%,and 31.92%,respectively.A small amount of air supply can improve the phosphorus removal efficiency of the system,while no air supply or more air supply can inhibit some microorganisms.Compared with the composition of the microbial community in each adsorbent column,it was found that a variety of microorganisms were proved,which have the functions of metabolizing organic matter,denitrifying nitrogen and phosphorus.The dominant bacteria with relatively high abundance on the phylum level include Proteobacteria(7.04%~70.33%),Firmicutes(0.91%~47.13%),Actinobacteria(9.06%~55.47%),and Bacteroidetes(6.49%~22.03%).The changing trend of the abundance of Proteobacteria was positively correlated with the removal rate of Bacteroidetes.The dominant bacteria with relatively high abundance on the genus level include Burkholderia(0.01%~31.5%),Clostridium_sensu_stricto(0.45%~34.47%),Pandoraea(0.63%~17.11%),Sphingomonas(0.48%~18.83%),Corynebacterium(6.36%~53.41%),Rhodobacter(0.01%~18.82%),and unclassified_Propionibacteriaceae(0.48%~34.91%

关 键 词：吸附 生物降解 除磷 氧环境 微生物群落 

分 类 号：TQ424[化学工程] X703.1[环境科学与工程—环境工程]