NDV基因Ⅶ型DNA纳米颗粒疫苗初探  

Preliminary Study on Gene Ⅶ DNA Nanoparticle Vaccine against Newcastle Disease Virus

作　　者：吴耀棠 梁健鹏 林思娴 刘宏梽 向斌[1] 丁铲[2] 徐成刚[1] 廖明[1] 任涛[1] WU Yaotang;LIANG Jianpeng;LIN Sixian;LIU Hongzhi;XIANG Bin;DING Chan;XU Chenggang;LIAO Ming;REN Tao(College of Veterinary Medicine,South China Agricultural University,Guangzhou 510642,China;Shanghai Veterinary Research Institute,CAAS,Shanghai 200241,China)

机构地区：[1]华南农业大学兽医学院,广州510642 [2]中国农业科学院上海兽医研究所,上海200241

出　　处：《中国动物传染病学报》2022年第2期34-41,共8页Chinese Journal of Animal Infectious Diseases

基　　金：国家重点研发计划种禽场新城疫鉴别诊断和监测技术集成与示范研究(2016YFD0501603)。

摘　　要：本研究利用PCR方法扩增新城疫病毒(NDV)rGM株的F基因,并将其克隆至真核表达载体pCAGGS,构建重组表达质粒pCAGGS-F。采用离子交联法将壳聚糖、MgSO4、pCAGGS-F以及三聚磷酸钠(TPP)混合,制备成新城疫DNA纳米颗粒疫苗。IFA试验检测结果表明,F重组蛋白成功表达。制备的纳米颗粒疫苗通过点眼滴鼻免疫2周龄SPF鸡2次后,对10^(3) EID_(50) NDV强毒株rGM保护率可达100%。此试验为新城疫DNA纳米疫苗等的研制奠定基础。In this study,the structural protein F gene of Newcastle disease virus(NDV)strain rGM was amplified by PCR and the PCR products were cloned into eukaryotic expression vector pCAGGS.DNA nanoparticle vaccine was then prepared by mixing of PCAGGS-F,chitosan,sodium tripolyphosphate(TPP)and ion crosslinking.The expression of the F gene was demonstrated in IFA.Twoweek-old SPF chickens were intranasally administered twice with the nanoparticle vaccine preparation and then challenged with 10^(3) EID_(50) NDV virulent strain rGM.As a result,all birds were protected.This experiment provided a promising prospect for the development of NDV DNA nanoparticle vaccine.

关 键 词：新城疫病毒 F基因 壳聚糖 纳米颗粒 

分 类 号：S858.65[农业科学—临床兽医学]