1株柔嫩艾美耳球虫ITS-1部分序列测定与分析  

Sequencing and Phylogenetic Analysis of ITS-1 from One Strain of Eimeria tenella

作　　者：魏冬霞[1] 袁橙 刘剑华 薛咏佩 WEI Dongxia;YUAN Cheng;LIU Jianhua;XUE Yongpei(The School of Veterinary Medicine,Jiangsu Agrianimal Husbandry Vocational College,Taizhou 225300,China)

机构地区：[1]江苏农牧科技职业学院动物医学院,泰州225300

出　　处：《中国动物传染病学报》2022年第2期147-152,共6页Chinese Journal of Animal Infectious Diseases

基　　金：江苏省高等学校自然科学研究面上项目(17KJB230003);江苏农牧科技职业学院项目(NSF201605);江苏省大学生创新创业训练计划(201912806010Y)。

摘　　要：根据已发表的柔嫩(Eimeria tenella)、堆型(E.acervulina)、布氏(E.brunetti)、巨型(E.maxima)、和缓(E.mitis)、毒害(E.necatrix)、早熟(E.praecox)艾美耳球虫的ITS-1序列引物,对江苏泰州地区某养鸡场发病鸡粪便中分离的球虫卵囊进行PCR扩增、测序,并用DNAStar软件和MEGA5.05软件对获得的艾美耳球虫ITS-1序列与GenBank中的艾美耳球虫的ITS-1相应序列进行同源性分析和系统进化关系分析。结果显示,柔嫩艾美耳球虫的引物扩增出阳性条带,该分离株的ITS-1部分序列片段长度为279 bp,与已发表的柔嫩艾美耳球虫的ITS-1序列的相似性为99.6%~100%,在构建的系统发育进化树中和柔嫩艾美耳球虫形成一个小分支。The oocysts were collected from the feces of diseased chickens on a farm in Taizhou,Jiangsu.Then the partial sequence of the ITS-1 regions of the Eimeria TZ strain isolated from these oocysts were amplified by PCR using the published ITS-1 sequence primers of E.tenella,E.acervulina,E.brunetti,E.maxima,E.mitis,E.necatrix and E.praecox.The phylogenetic relationship between ITS-1 sequences of this Eimeria TZ strain and the reference strains in GenBank was analyzed using DNAStar software and MEGA5.05 software.The results showed that a positive band was obtained when primers of E.tenella were used in the PCR amplification.Therefore,the TZ strain was determined to be E.tenella.The length of ITS-1 fragment of E.tenella TZ strain was 279 bp and its similarity with other published E.tenella strains was 99.6%-100%.A small E.tenella branch of the phylogenetic evolutionary tree formed including this TZ strain and other E.tenella strains.

关 键 词：柔嫩艾美球虫 ITS-1 系统发育分析 

分 类 号：S852.723[农业科学—基础兽医学]