马驽巴贝斯虫ddPCR 检测方法的建立  

Development of ddPCR for Detection of Babesia caballi

作　　者：陈少博 杨帆 荆文魁 王伊琴 康晓平 常鸿 聂芝君 CHEN Shaobo;YANG Fan;JING Wenkui;WANG Yiqin;KANG Xiaoping;CHANG Hong;NIE Zhijun(Technology Center of Erenhot Costoms,Erenhot 011100,China;State Key Laboratory of Pathogen and Biosecurity,Institute of Microbiology and Epidemiology,Academy of Military Medical Sciences,Beijing 100071,China)

机构地区：[1]二连海关技术中心,二连浩特011100 [2]军事科学院军事医学研究院微生物流行病研究所,北京010071

出　　处：《中国动物传染病学报》2022年第2期153-158,共6页Chinese Journal of Animal Infectious Diseases

基　　金：原内蒙古检验检疫局自主立项科技项目(NMCIQ-IK2017-001);海关总署科研计划项目(2019HK042);传染病重大专项(2018zx10711001-003-001)。

摘　　要：马驽巴贝斯虫(Babesia caballi)病是马属动物的一种血液原虫病,被农业农村部列为二类动物疫病。本研究旨在建立一种针对马驽巴贝斯虫病的ddPCR检测技术。利用马驽巴贝斯虫特异性引物及探针对反应体系进行优化,测试了方法的特异性、稳定性及灵敏性,对30份可疑样品进行了初步检测。结果显示,该方法具有较好的稳定性,最低定量检测限可达3.3 copies/μL,对30份样品进行检测,马驽巴贝斯虫病阳性率为63.33%(19/30)。表明所建立的方法可成功用于马驽巴贝斯虫的检测。Babesia caballi is one of the etiological agents of equine babesiosis,which was listed as the second-class epidemic disease by Ministry of Agriculture and Rural Affairs of China.In this study,a ddPCR method was developed for detection of B.caballi infection by using a pair of specific primers and a probe.This method was tested for optimal specificity,stability and sensitivity.The results showed that this method was significantly reproducible,and its detection limit was 3.3 copies/μL,In addition,30 suspicious blood samples were tested using this method and the positive rate was 63.33%(19/30)for B.caballi.

关 键 词：马驽巴贝斯虫 ddPCR 检测 

分 类 号：S858.21[农业科学—临床兽医学]