基于Pancharatnam-Berry相位透镜的三维单粒子定位方法研究  

Research of three-dimensional single particle localization method based on Pancharatnam-Berry phase lense

作　　者：曹博[1,2] 霍英东[1,4] 曹慧群 于斌 CAO Bo;HUO Yingdong;CAO Huiqun;YU Bin(Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province,College of Physics and Optoelectronic Engineering,Shenzhen University,Shenzhen Guangdong 518060,China;College of Electronics and Information Engineering,Shenzhen University,Shenzhen Guangdong 518060,China;College of Chemistry and Environmental Engineering,Shenzhen University,Shenzhen Guangdong 518060,China;Nanchang Virtual Reality Institute Co.,Ltd,Nanchang 330000,China)

机构地区：[1]深圳大学物理与光电工程学院,光电子器件与系统教育部/广东省重点实验室,广东深圳518060 [2]深圳大学电子与信息工程学院,广东深圳518060 [3]深圳大学化学与环境工程学院,广东深圳518060 [4]南昌虚拟现实研究院股份有限公司,南昌330000

出　　处：《激光杂志》2022年第4期31-35,共5页Laser Journal

基　　金：国家自然科学基金(No.62175166,61975131);深圳市基础研究项目(No.JCYJ20200109105411133,JCYJ20180305125649693);中国博士后科学基金(No.2018M643154)。

摘　　要：在三维单粒子追踪中,现有的双焦面轴向定位方法光路复杂,光利用率低,针对这一问题,提出了一种基于Pancharatnam-Berry相位透镜的三维单粒子定位方法。通过在传统荧光显微成像系统的光路中加入具有正负双焦距特性的Pancharatnam-Berry相位透镜,可以实现两个焦面同时成像在一个探测器上,利用在焦平面的光斑图像定位可获得单粒子的横向(x-y方向)位置信息,利用离焦平面与在焦平面的光斑强度比可以获得单粒子的轴向(z方向)位置信息。实验上,采用±100 mm焦距的Pancharatnam-Berry相位透镜,对1μm的荧光珠进行三维位置定位,获得了x,y,z方向分别为10 nm, 9 nm, 22 nm的位置定位精度。A new 3 D single particle localization method based on Pancharatnam-Berry phase is proposed considering that the complex imaging system and low optical efficiency in bifocal imaging method for three-dimensional particle tracking. Two focal planes could be simultaneously imaged on the same detector through adding a Pancharatnam-Berry phase lens with positive and negative focal length to the optical path of the traditional fluorescence microscopy system. The lateral(X-Y) position of particles could be obtained by fitting the center of the spot in the focal plane. The axial(Z direction) position of particles could be obtained by using the intensity ratio between the focus spot and the defocal spot in the same imaging plane. In the experiment, a Pancharatnam-Berry phase lens with a focal length of ±100 mm was used to locate 1 μm fluorescent beads in three dimensions, and the positioning accuracy of X, Y and Z directions is 10 nm, 9 nm and 22 nm, respectively.

关 键 词：Pancharatnam-Berry相位 荧光显微成像 双焦面成像 三维单粒子定位 

分 类 号：TN27[电子电信—物理电子学]