基于近红外荧光标记蛋白芯片的多肿瘤标志物联合定量检测技术  

Combined quantitative detection technology for multiple tumor markers based on near-infrared fluorescent labeled protein chip

作　　者：王志慧 项华中[1] 郑刚[1] 许颖原 WANG Zhihui;XIANG Huazhong;ZHENG Gang;XU Yingyuan(Shanghai Research Engineering Center of Interventional Medical Device,University of Shanghai for Science and Technology,Shanghai 200093,China;Shanghai Saga Biological Technology Co.,Ltd.,Shanghai 201203,China)

机构地区：[1]上海理工大学上海市介入医疗器械工程研究中心,上海200093 [2]上海萨迦生物科技有限公司,上海201203

出　　处：《光学仪器》2022年第2期7-14,共8页Optical Instruments

基　　金：国家自然科学基金青年科学基金(61605114)。

摘　　要：为了有效降低恶性肿瘤的死亡率,提高肿瘤筛查的普适性、稳定性和灵敏度,建立了近红外荧光标记蛋白芯片的多肿瘤标志物联合定量检测系统。对该系统的近红外荧光标记蛋白芯片免疫分析原理和用于蛋白芯片扫描分析的生物芯片扫描仪进行了研究。采用双抗体夹心的近红外荧光法检测,在固相载体上包被多种肿瘤标志物抗体,捕获被检者血清中对应肿瘤标志物的抗原,再结合生物素标记的二抗,并与近红外荧光标记的链霉亲和素结合,利用专门研制的生物芯片扫描仪进行扫描分析,进而获得被检者血清中肿瘤标志物含量的生物信息。最后,利用该检测系统和罗氏电化学发光免疫分析仪对10例样本血清中4项肿瘤标志物进行检测。实验结果表明,两仪器对肿瘤标志物CA125、CEA、CA19-9、AFP检测的线性相关系数分别为0.995、0.992、0.991和0.990。近红外荧光标记蛋白芯片的多肿瘤标志物联合定量检测系统可实现多人份多项肿瘤标志物的一次性检测,结果与罗氏单人份单指标检测结果高度相关。In order to effectively reduce the mortality of malignant tumors and improve the universality,stability and sensitivity of tumor screening,a combined quantitative detection system for multiple tumor markers with near-infrared fluorescent-labeled protein chips has been established.The principle of near-infrared fluorescent-labeled protein chip immunoassay of the system and the biochip scanner used for protein chip scanning and analysis were studied.A double-antibody sandwich near-infrared fluorescence detection method was used.By coating a variety of tumor marker antibodies on the solid-phase carrier,the antigen corresponding to the tumor marker in the serum of the subject was captured.The reactant was first combined with biotin-labeled secondary antibody,and then combined with near-infrared fluorescent-labeled streptavidin.A specially developed biochip scanner was used for scanning analysis to obtain biological information on the content of tumor markers in the examinee's serum.Finally,the detection system and Roche electrochemiluminescence equipment were used to detect 4 tumor markers in the serum of 10 samples.The experimental results show that the linear correlation coefficients of the detection results of the tumor markers CA125,CEA,CA19-9 and AFP by the two instruments are 0.995,0.992,0.991 and 0.990.The multi-tumor markers combined quantitative detection system of the near-infrared fluorescent-labeled protein chip can realize the simultaneous detection of multiple tumor markers for multiple persons,and the results are highly correlated with the results of Roche single-person single-index detection.

关 键 词：近红外荧光 蛋白芯片 多肿瘤标志物 定量检测 

分 类 号：TH773[机械工程—仪器科学与技术] R730.45[机械工程—精密仪器及机械]