污水中包膜病毒离心超滤浓缩方法研究  

Concentration of Enveloped Viruses in Wastewater by Centrifugal Ultrafiltration Method

作　　者：李方旭 杨伊菡 周小红[1] 杨云锋[1] 刘锐平 陆韻[1] 刘艳臣[1] 黄霞[1] LI Fang-xu;YANG Yi-han;ZHOU Xiao-hong;YANG Yun-feng;LIU Rui-ping;LU Yun;LIU Yan-chen;HUANG Xia(School of Environment,Tsinghua University,Beijing 100084,China)

机构地区：[1]清华大学环境学院,北京100084

出　　处：《中国给水排水》2022年第7期28-32,共5页China Water & Wastewater

基　　金：国家自然科学基金资助重大项目(52091541)。

摘　　要：针对污水中包膜病毒高效富集浓缩方法缺乏的问题,以Phi6噬菌体为模式包膜病毒,系统考察了超滤浓缩方法对污水样本中Phi6噬菌体的富集浓缩效果。结果表明,超滤筒的截留分子质量越小,对污水中Phi6噬菌体的回收率就越高,但所需浓缩时间也会增加。综合考虑回收率和耗时,推荐采用截留分子质量为10 ku的超滤筒,在4℃条件下3000g正向离心28 min、1000g反向收集5 min,浓缩倍数为110倍,回收率可达到(32±3.7)%。In view of the lack of methods for efficient concentration of enveloped viruses,this paper systematically investigated the performance of centrifugal ultrafiltration method for concentration of the bacteriophage Phi6(taking it as a model enveloped virus)in wastewater.The recovery rate of Phi6increased with the decrease of molecular weight cut-off of the ultrafiltration cartridge,however,accompanying with the prolonged concentration time.Considering the recovery performance and time,it was recommended to select the ultrafiltration cartridge with the molecular weight cut-off of 10 ku.Under the conditions of 4℃,forward centrifugation of 3000g for 28 min and backward centrifugation of 1000g for 5 min,and 110 times of concentration ratio,the recovery rate reached(32±3.7)%.

关 键 词：包膜病毒 污水 超滤 浓缩 

分 类 号：TU992[建筑科学—市政工程]