水稻Os SSRP的生物信息学分析及耐盐性研究  

Bioinformatics analysis and salt tolerance studies on the OsSSRP of rice

作　　者：曾佳 刘玲 周天顺 荣秀琴 袁定阳[4] 段美娟[1,2] ZENG Jia;LIU Ling;ZHOU Tianshun;RONG Xiuqin;YUAN Dingyang;DUAN Meijuan(College of Bioscience and Biotechnology,Hunan Agricultural University,Changsha,Hunan 410128,China;College of Agronomy,Hunan Agricultural University,Changsha,Hunan 410128,China;Long Ping Branch,Graduate School of Hunan University,Changsha,Hunan 410125,China;Hunan Hybrid Rice Research Center,Changsha,Hunan 410125,China)

机构地区：[1]湖南农业大学生物科学技术学院,湖南长沙410128 [2]湖南农业大学农学院,湖南长沙410128 [3]湖南大学研究生院隆平分院,湖南长沙410125 [4]湖南杂交水稻研究中心,湖南长沙410125

出　　处：《湖南农业大学学报（自然科学版）》2022年第2期151-159,共9页Journal of Hunan Agricultural University(Natural Sciences)

基　　金：国家自然科学基金面上项目(31771767);湖南省科技人才托举工程项目(2019TJ–Q08);湖南省科技重大专项(2018NK1010)。

摘　　要：为鉴定水稻RR(response regulators)家族成员OsSSRP(salt–sensitive RR protein,LOC_Os08g35670)是否响应盐胁迫,以日本晴为背景材料,采用Blast比对、结构域预测和启动子元件分析等方法,分析发现Os SSRP仅含REC结构域基序,其启动子元件中含有大量脱落酸和茉莉酸响应元件;诱导表达谱分析发现,Os SSRP响应盐和干旱逆境胁迫及细胞分裂素、脱落酸和茉莉酸诱导;利用CRISPR/Cas9技术定向敲除后获得纯合突变体ssrp,通过0、40、100 mmol/L NaCl进行种子萌发期盐胁迫处理和150 mmol/L NaCl的苗期耐盐性鉴定,发现纯合突变体ssrp对盐胁迫更为敏感,盐胁迫后纯合突变体ssrp存活率远远低于野生型植株。说明OsSSRP与非生物胁迫、激素响应密切相关,可为水稻非生物胁迫抗性育种提供基因资源。To identify whether the RR family member Os SSRP(salt-sensitive RR Protein,LOC_Os08g35670)of rice was response to salt stress,using the nipponbare as the material,we employed bioinformatics analysis including gene blast,domain prediction and promoter analysis of their sequences.It showed that Os SSRP contained only REC domain motif,and its promoter elements possessed large number of abscisic and jasmonic acid responsive elements.Induced expression profile analysis found that Os SSRP responded to salt and drought stress and induction of cytokinin,abscisic acid,and jasmonic acid.After seedling stage identification under 0,40,100 mmol/L NaCl and seed germination stage identification under 150 mmol/L NaCl,it was found that the homozygous mutant ssrp targeted knockout using CRISPR/Cas9 technology was more sensitive to salt stress.The mutant survival rate of salt stress was much lower than that of the wild type.Os SSRP was closely related to abiotic stress and hormone response,providing potential gene resources for abiotic stress resistance breeding in the rice.

关 键 词：水稻 激素响应 RR家族基因 生物信息学 耐盐性 

分 类 号：S511.01[农业科学—作物学]