基于EST-SSR标记鉴别人参、西洋参和三七的研究  

Identification of Panax ginseng,Panax quinquefolius and Panax notoginseng by EST-SSR markers

作　　者：范馨元 王添琦 惠赫童 武伦鹏 林成日 刘丽梅 FAN Xin-yuan;WANG Tian-qi;HUI He-tong;WU Lun-peng;LIN Cheng-ri;LIU Li-mei(College of Medical Technology,Beihua University,Jilin 132013,China;National Ginseng Products Quality Supervision Inspection Center,Yanbian 133000,China;Jilin Yisheng Hanshen Biotechnology Co.,Ltd,Baishan 134521,China)

机构地区：[1]北华大学医学技术学院,吉林132013 [2]国家参茸产品质量监督检验中心,延边133000 [3]吉林省益盛汉参生物科技有限公司,白山134521

出　　处：《药物分析杂志》2022年第3期394-401,共8页Chinese Journal of Pharmaceutical Analysis

基　　金：吉林省科技发展计划项目(20190304108YY)资助。

摘　　要：目的:应用简单重复序列(SSR)标记对人参属植物人参、西洋参和三七进行鉴别。方法:利用EGassembler对NCBI的EST数据库进行预处理,SSRIT网站寻找SSR位点后用Primer3设计特异性引物。采用非变性聚丙烯酰胺凝胶电泳和毛细管电泳2种方法筛选并验证EST-SSR引物的通用性。应用筛选的SSR引物构建标准指纹图谱并对市售商业参类产品的原材料进行鉴定。结果:应用生物信息学的方法共筛选出7对EST-SSR标记可以用于人参、西洋参和三七的鉴定。在市售商业产品的检测中,以引物P2和Q11的联合检测阳性率最高。结论:SSR标记具有较高的重复性和准确性,可以作为人参属植物可靠的分子标记。Objective:Using the simple sequence repeat(SSR)markers to identify Panax ginseng,Panax quinquefolium and Panax notoginseng.Methods:The EST database of NCBI was pre-processed by using EGassembler.SSRIT website was used to identify SSR loci and Primer3 was used to design specific primers.The universality and polymorphism of EST-SSR primers were screened and verified by non-denatured polyacrylamide gel electrophoresis and capillary electrophoresis.These SSR primers were used to construct standard fingerprints and identify the raw materials of ginseng products from the market.Results:Through bioinformatics methods,seven pairs of EST-SSR markers were screened for identification of Panax ginseng,Panax quinquefolium and Panax notoginseng.The combination of primers P2 and Q11 showed the highest positive rate in the detection of commercial products.Conclusion:SSR molecular markers are repeatable and accurate,which can be used as reliable molecular markers for identification of Panax species.

关 键 词：表达序列标签(SSR) 人参 西洋参 三七 鉴别 

分 类 号：R917[医药卫生—药物分析学]