木质素降解真菌菌群LDFC产酶条件优化  

Optimization of Enzyme-producing Conditions of Lignin-degrading Fungal Flora LDFC

作　　者：王敬红 李灵灵 申贵男 袁媛 曹迪[1] 高亚梅[1] 晏磊[1,2] 王伟东[1,2] Wang Jinghong;Li Lingling;Shen Guinan;Yuan Yuan;Cao Di;Gao Yamei;Yan Lei;Wang Weidong(Heilongjiang Provincial Key Laboratory of Environmental Microbiology and Recycling of Argo-waste in Cold Region/College of Life Science and Technology,Heilongjiang Bayi Agricultural University,Daqing163319;Engineering Research Center of Processing and Utilization of Grain By-products,Ministry of Educatio.)

机构地区：[1]黑龙江省寒区环境微生物与农业废弃物资源利用重点实验室/黑龙江八一农垦大学生命科学技术学院,大庆163319 [2]粮食副产物加工与利用教育部工程中心

出　　处：《黑龙江八一农垦大学学报》2022年第2期75-80,115,共7页journal of heilongjiang bayi agricultural university

基　　金：黑龙江省自然科学基金重点项目(ZD2018005);国家重点研发计划(2018YFD0800906-03);中央财政支持地方发展科技项目(ZY16A06-02);黑龙江省高校科技创新团队项目(2012TD006);黑龙江农垦总局科技攻关项目(HKKY190404);黑龙江八一农垦大学科技创新团队项目(TDJH201809);黑龙江八一农垦大学研究生创新项目(YJSCX2019-Y62)。

摘　　要：把菌株BYL-3(Trametes hirsuta)、BYL-7(Trametes versicolor)和BYL-8(Trametes hirsuta)三株具有木质素分解能力的真菌混合培养,构建了具有更高木质素降解能力的真菌菌群LDFC。为获得菌群LDFC的最佳产酶条件,从碳源种类、氮源种类、Cu^(2+)浓度、Mn^(2+)浓度和诱导剂种类五个方面进行优化。LDFC的最佳产酶条件为添加麦芽糖为复合碳源,硝酸钠为氮源,Cu^(2+)浓度为1.5 mmol·L^(-1),Mn^(2+)浓度为0.2 mmol·L^(-1),ABTS为诱导剂,优化后漆酶(Lac)、木质素过氧化物酶(Lip)、锰过氧化物酶(Mnp)的酶活性分别为678.3、1463.6、2667.8 U·L^(-1),与优化前相比分别提高142.8%、49.0%、110.0%。试验为利用多菌株协同降解木质素的研究和后续应用提供数据支持。Three fungi with lignin decomposing ability,BYL-3(Trametes hirsuta),BYL-7(Trametes versicolor)and BYL-8(Trametes hirsuta),were mixed and cultured to construct a fungal complex strain LDFC with higher degradability.The experiment optimized the enzyme production conditions of the LDFC from five aspects:carbon source type,nitrogen source type,Cu^(2+)conc-entration,Mn^(2+) concentration and inducer type.The experimental results showed that the best enzyme production conditions for LDFC were as follows:adding maltose as a composite carbon source,sodium nitrate(SN)as a nitrogen source,the Cu^(2+)concentration of 1.5 mmol·L^(-1),the Mn^(2+)concentration of 0.2 mmol·L^(-1)and ABTS be the inducer.After optimization,the enzyme activities of laccase(Lac),lignin peroxidase(Lip)and manganese peroxidase(Mnp)were 678.3 U·L^(-1),1463.6 U·L^(-1),and 2667.8 U·L^(-1),respectively.Compared with non-optimized,it increased by 142.8%,49.0%and 110.0%respectively.The experiment provided data support for the study and subsequent application of lignin degradation by multi-strain collaboration.

关 键 词：真菌 混合培养 木质素降解 酶 条件优化 

分 类 号：Q93[生物学—微生物学]