Humulus japonicus extract alleviates oxidative stress and apoptosis in 6-hydroxydopamine-induced PC12 cells  

作　　者：Feng Wang Byoung Ok Cho Jae Young Shin Suping Hao Seon Il Jang 

机构地区：[1]Department of Health Management,Jeonju University,Jeonju 55069,Korea [2]Research Institute,Ato Q&A Co,LTD,Cheonjam-ro,Wansan-gu,Jeonju 55069,Korea [3]Department of Life Sciences,Yuncheng University,Yuncheng,Shanxi Province 044000,PR China [4]Institute of Health&Science,Jeonju University,Jeonju 55069,Korea

出　　处：《Asian Pacific Journal of Tropical Biomedicine》2022年第5期197-206,共10页亚太热带生物医学杂志（英文版）

基　　金：financially supported by the Ministry of Small and Medium Enterprise and Startups (MSS),Korea,under the Social Economy Innovation Growth Project (R&D)(Project number P0013037)

摘　　要：Objective:To explore the possible neuroprotective activities of Humulus japonicus extract against Parkinson's disease(PD)in a cellular model.Methods:PD was modeled in PC12 cells using 6-hydroxydopamine(6-OHDA).The cell activity,intracellular levels of reactive oxygen species(ROS),anti-oxidative and anti-apoptotic effects,and other related indicators and related signaling pathways were evaluated to elucidate the neuroprotective effects of Humulus japonicus extract.Results:Humulus japonicus extract exhibited anti-oxidative and anti-apoptotic effects in 6-OHDA-stimulated PC12 cells.It also reduced oxidative stress-induced ROS accumulation;upregulated antioxidant enzymes,such as glutathione,catalase,heme oxidase-1,and 8-oxguanine glycosylase 1;promoted cell survival by decreasing BAX and increasing Bcl-2 and sirtuin 1 expression via the MAPK and/or Nrf2 signaling pathways.Conclusions:Humulus japonicus extract has antioxidative and anti-apoptotic effects and could be developed as a promising candidate for preventing and treating oxidative stress-related neurodegenerative diseases.

关 键 词：Humulus japonicus Parkinson’s disease Oxidative stress APOPTOSIS MAPK PC12 cells Nrf2 

分 类 号：R285[医药卫生—中药学]