中药渣堆肥微生物群落结构及纤维素降解酶基因表达量变化特征  

Change characteristics of microbial community structure and cellulose degrading enzyme gene expression level in Chinese medicine residue compost

作　　者：杜婕 宋修超[1,2,3] 马艳 DU Jie;SONG Xiu-chao;MA Yan(Institute of Agricultural Resources and Environment,Jiangsu Academy of Agricultural Sciences,Nanjing 210014,China;Key Laboratory of Agro-Environment in Downstream of Yangtze Plain,Ministry of Agriculture and Rural Affairs,Nanjing 210014,China;School of the Environment and Safety Engineering,Jiangsu University,Zhenjiang 212013,China;Institute of Animal Husbandry and Veterinary Medicine,Jiangsu Vocational College of Agriculture and Forestry,Zhenjiang 212400,China)

机构地区：[1]江苏省农业科学院农业资源与环境研究所,江苏南京210014 [2]农业农村部长江下游农业环境重点实验室,江苏南京210014 [3]江苏大学环境与安全工程学院,江苏镇江212013 [4]江苏农林职业技术学院畜牧兽医学院,江苏镇江212400

出　　处：《江苏农业学报》2022年第2期352-360,共9页Jiangsu Journal of Agricultural Sciences

基　　金：国家重点研发计划项目(2017YFD0800200);江苏省农业科技自主创新基金项目[CX(17)2025];中国博士后科学基金项目(2018M632255);江苏农林职业技术学院青年支持项目(2020kj011)。

摘　　要：本研究对不同堆制时期的中药渣堆肥样品进行Miseq高通量测序分析,并对8种纤维素降解酶基因进行荧光定量RT-PCR分析,以解析中药渣堆肥过程中微生物群落结构组成及纤维素降解酶基因表达丰度的变化特征。结果表明,在中药渣堆肥过程中,不同堆制时期的微生物群落结构组成不同,并且不同处理间的群落结构也存在差异。对样品中微生物群落进行主成分分析(PCoA),发现在中药渣堆肥过程中,不同处理的组间微生物群落结构存在较大差异,组内群落结构不断变化并逐渐趋于稳定。荧光定量RT-PCR检测结果显示,在中药渣堆肥过程中,纤维素降解酶基因的表达丰度也处于明显的变化中。说明,中药渣堆肥中微生物群落与纤维素降解酶是相互联系、相互影响的。In this study,Miseq high-throughput sequencing analysis was performed on samples of Chinese medicine residue compost during different composting periods,and eight kinds of cellulose degrading enzyme genes were analyzed by fluorescence quantitative RT-PCR to explore the composition of microbial community structure and the expression and abundance of cellulose degrading enzyme genes during the composting of Chinese medicine residues.Results showed that during the composting of Chinese medicine residues,the composition of microbial community structure in different periods was different,and the community structure was also different in different treatments.Principal component analysis(PCoA)results indicated that during the composting of Chinese medicine residues,there were significant differences in the microbial community structure among different treatments,and the community structure within the group changed continuously and gradually stabilized.Results of fluorescence quantitative RT-PCR showed that the expression abundance of cellulose degrading enzyme genes also changed significantly during the composting of Chinese medicine residues.In conclusion,the microbial community and cellulose degrading enzymes in Chinese medicine residue compost are interrelated and interacted.

关 键 词：中药渣堆肥 Miseq高通量测序 群落结构 纤维素降解酶基因 

分 类 号：S141.4[农业科学—肥料学]