薏苡仁多糖通过调控JAK3/STAT5通路改善细胞免疫功能的体内外研究  

In vitro and in vivo study of coixan improving cellular immune function by regulating the JAK3/STAT5 pathway

作　　者：王彦芳[1] 杨彬彬[1] 陈倩[1] 崔宁[1] 赵文晓[1] 武继彪[1] WANG Yan-fang;YANG Bin-bin;CHEN Qian;CUI Ning;ZHAO Wen-xiao;WU Ji-biao(Shandong University of Traditional Chinese Medicine,Jinan 250355,China)

机构地区：[1]山东中医药大学,济南250355

出　　处：《中华中医药杂志》2021年第11期6414-6417,共4页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家重点基础研究发展计划(973计划)(No.2013CB531803);国家自然科学基金青年科学基金项目(No.81803864);山东省自然科学基金面上项目(No.ZR2020MH395);山东省中医药科技发展计划项目(No.2019-0052)。

摘　　要：目的:研究薏苡仁多糖及其组分对大鼠脾淋巴细胞增殖的影响及对脾虚水湿不化(DSSD)大鼠免疫功能改善的机制。方法:通过CCK-8法检测薏苡仁多糖及其组分对脾淋巴细胞增殖的影响,采用Western Blot检测薏苡仁多糖及其组分对DSSD大鼠结肠组织JAK3、STAT5的磷酸化水平。结果:62.5~250μg/mL的粗多糖、250~4 000μg/mL的多糖组分Ⅰ、62.5~1 000μg/mL的多糖组分Ⅱ在体外能显著促进淋巴细胞增殖,薏苡仁多糖及其拆分组分能够上调DSSD大鼠的结肠组织JAK3、STAT5磷酸化水平。结论:薏苡仁多糖及其组分可改善细胞免疫功能,其机制可能与激活IL-2介导的JAK3/STAT5信号通路,促进T淋巴细胞增殖有关。Objective: To study the effects of coixan and its components on the proliferation of rat spleen lymphocytes and to explore the mechanisms of coixan and its components on immune system improvement in TCM syndrome of dampness stagnancy due to spleen deficiency(DSSD) rat. Methods: CCK-8 method was used to detect the proliferation of spleen lymphocytes, and Western Blot was used to detect the phosphorylation levels of JAK3 and STAT5 in colon tissues of DSSD rats.Results: Crude polysaccharide(62.5~250μg/mL), polysaccharideⅠ(250~4 000μg/mL), polysaccharide Ⅱ(62.5~1 000μg/mL)could significantly promote the proliferation of lymphocytes. Coixan and its components could also up-regulate the JAK3 and STAT5 phosphorylation levels in colon tissues of DSSD rats. Conclusion: Coixan can improve cellular immune function, and the mechanism may be related to the activation of IL-2-mediated JAK3/STAT5 pathway and the promotion of T lymphocyte proliferation.

关 键 词：薏苡仁多糖 免疫功能 JAK3/STAT5通路 体内外研究 

分 类 号：R285.5[医药卫生—中药学]