青蒿-鳖甲药对配伍对系统性红斑狼疮小鼠肠道菌群的调节作用  

Regulating effect of Qinghao-Biejia on gut microbiota in the treatment of MRL/lpr mice

作　　者：林笑颖 余怡然 刘秋萍[1] 何志兴[1] 李海昌[1] 温成平[1] LIN Xiao-ying;YU Yi-ran;LIU Qiu-ping;HE Zhi-xing;LI Hai-chang;WEN Cheng-ping(Institute of Basic Research in Clinical Medicine,College of Basic Medical Sciences,Zhejiang Chinese Medical University,Hangzhou 310053,China)

机构地区：[1]浙江中医药大学基础医学院中医临床基础研究所,杭州310053

出　　处：《中华中医药杂志》2021年第11期6743-6746,共4页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家自然科学基金项目(No.82074217,No.81973829);浙江省自然科学基金项目(No.LY21H270006)。

摘　　要：目的:探究青蒿-鳖甲治疗系统性红斑狼疮(SLE)小鼠的效果,并从肠道菌群角度分析可能的机制。方法:运用Illumina MiSeq高通量测序技术检测MRL/lpr小鼠的肠道菌群结构,利用LEfSe分析挖掘青蒿-鳖甲改变的肠道菌群种类,采用Spearman方法对狼疮指标及改变的肠道菌群进行关联性分析。结果:高剂量青蒿-鳖甲治疗MRL/lpr小鼠的效果与激素相当,其能通过增加肠道菌群Alpha多样性指数,上调与狼疮病情显著负相关的菌属(Bacteroides、Parabacteroides和Odoribacter)和下调与狼疮病情显著正相关的菌属(Clostridium和Ruminococcus),达到治疗狼疮的目的。结论:青蒿-鳖甲治疗SLE时显著改变的肠道菌群,利于狼疮病情的缓解。Objective: To explore the therapeutic effect of Qinghao-Biejia in the treatment of MRL/lpr mice and reveal the possible mechanism from the perspective of gut microbiome. Methods: Illumina MiSeq sequencing was used to explore the microbial communities of MRL/lpr mice. LEfSe analysis was used to reveal the significantly altered gut microbiota by Qinghao-Biejia and Spearman method was employed to correlate the altered gut microbiota and lupus indices. Results: The therapeutic effect of high-dose Qinghao-Biejia was consistent with that of glucocorticoid. In addition, Qinghao-Biejia could increase Alpha diversity and negatively lupus activity-related microbial genus including Bacteroides, Parabacteroides and Odoribacter, and decrease positively lupus activity-related microbial genus including Clostridium and Ruminococcus. Conclusion: The significantly altered gut microbiota genus by Qinghao-Biejia is beneficial to the remission of lupus.

关 键 词：MRL/lpr狼疮小鼠 青蒿-鳖甲 肠道菌群 Illumina MiSeq测序 

分 类 号：R285.5[医药卫生—中药学]