防粘汤对宫腔粘连大鼠TGF-β1/Smad信号通路的影响  

Effects of Fangzhan Decoction on TGF-β1/Smad signaling pathway in rats with intrauterine adhesions

作　　者：徐佳[1] 谭雅莉 彭壮 李泰 蒋冬 雷磊[2] 马本玲[1] 匡继林[5] 林丽美[3] XU Jia;TAN Ya-li;PENG Zhuang;LI Tai;JIANG Dong;LEI Lei;MA Ben-ling;KUANG Ji-lin;LIN Li-mei(The Second Affiliated Hospital of Hunan University of Chinese Medicine,Changsha 410005,China;School of the Integrated Traditional Chinese and Western Medicine,Hunan University of Chinese Medicine,Changsha 410208,China;School of Pharmaceutical Science,Hunan University of Chinese Medicine,Changsha 410208,China;Clinical Medical College of Hunan University of Chinese Medicine,Changsha 410208,China)

机构地区：[1]湖南中医药大学第二附属医院,长沙410005 [2]湖南中医药大学中西医结合学院,长沙410208 [3]湖南中医药大学药学院,长沙410208 [4]湖南中医药大学临床医学院,长沙410208 [5]湖南中医药大学第二附属医院妇科,长沙410005

出　　处：《中华中医药杂志》2021年第12期7350-7355,共6页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：湖南省教育厅一般科研项目(No.18C0376,No.19C1398);湖南中医药大学2018年度中西医结合一流学科开放基金项目(No.2018ZXYJH09);湖南省研究生科研创新项目(No.CX20200796);湖南中医药大学研究生创新课题(No.2019CX20)。

摘　　要：目的:探讨防粘汤对宫腔粘连大鼠的作用及其可能机制。方法:将76只SD大鼠随机分为正常组(12只)、假手术组(12只)及造模组(52只),采用机械刮宫及感染双重损伤建立宫腔粘连大鼠模型,造模成功后,将造模组随机分为模型组、雌激素组(0.63mg·kg^(-1)·d^(-1))及防粘汤低、中、高剂量(4.14、8.28、16.56g·kg^(-1)·d^(-1))组,各10只。连续灌胃给药21d后处死大鼠,收集其双侧子宫组织,肉眼观察其大体形态学改变,HE及Masson染色检测其组织病理学变化;RT-PCR及Western Blot法检测子宫组织中TGF-β1和Smad2、Smad3、Smad7 mRNA及蛋白表达。结果:与正常组比较,模型组大鼠子宫组织肿胀,弹性差,子宫指数增高,内膜有炎症细胞浸润,重度纤维化;子宫组织中TGF-β1和Smad2、Smad3 mRNA和蛋白表达显著升高,而Smad7mRNA和蛋白表达显著降低(P<0.01)。与模型组比较,防粘汤中、高剂量组大鼠子宫形态、色泽及柔韧性明显好转,子宫指数降低,炎症及纤维化程度均下降(P<0.01);子宫组织中TGF-β1和Smad2、Smad3 mRNA和蛋白表达显著降低,Smad7 mRNA和蛋白表达显著升高(P<0.05,P<0.01)。结论:防粘汤可能通过阻断TGF-β1/Smad信号通路延缓子宫内膜纤维化进程,促进内膜修复。Objective:To explore the treatment and pathogenesis of Fangzhan Decoction (FZD) in rats with intrauterine adhesion.Methods:Seventy-six SD rats were randomly divided into normal group (n=12),sham operation group (n=12) and model group (n=52).The rat models of intrauterine adhesions were established using mechanical injury and infection.After the establishment of the model,the modeling group were randomly divided into positive control group and FZD at a dose of4.14,8.28,16.56g·kg^(-1)·d^(-1) respectively daily for 21d.The rats were then sacrificed and uterine tissues were collected.Gross morphological changes were observed;The pathological changes and fibrosis of the uterine tissues were observed by HE staining and Masson staining;Real-time quantitative PCR and Western Blot method were used to detect the expression of key factors in TGF-β1/Smad signaling pathway in uterus tissue.Results:Compared with the control group,the uterus of the model group was swollen,distorted,with poor elasticity,the uterine/body coefficient was increased,the inflammation and fibrosis were aggravated.The levels of TGF-β1,Smad2,Smad3 mRNA and protein were significantly increased,while Smad7 was decreased markedly(P<0.01) in the model group.Compared with the model group,the uterus shape,color and flexibility of the rats in the medium and high dose groups were significantly improved,the uterine/body was decreased,the degree of inflammation and fibrosis were alleviated(P<0.01).The levels of TGF-β1,Smad2,Smad3 mRNA and protein were significantly decreased,and the levels of Smad7 mRNA and protein expression were significantly increased (P<0.05,P<0.01).Conclusion:FZD could slow down the process of uterine fibrosis,and promote the regeneration of endometrium,the mechanism may be related to block TGF-β1/Smad signaling pathway.

关 键 词：宫腔粘连 防粘汤 TGF-Β1/SMAD信号通路 纤维化 

分 类 号：R285.5[医药卫生—中药学]